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MiRNA marker for detecting brain injury, kit, application

A technology for markers and brain damage, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of lack of precision and sensitivity, achieve simplification, strong operability, and appropriate amount of targets , Good tissue-specific effect

Inactive Publication Date: 2019-01-25
成都仕康美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the severity and postoperative recovery of traumatic brain injury are mostly evaluated clinically through imaging observation and behavioral scoring, which lacks sufficient accuracy and sensitivity.

Method used

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  • MiRNA marker for detecting brain injury, kit, application
  • MiRNA marker for detecting brain injury, kit, application
  • MiRNA marker for detecting brain injury, kit, application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A diagnostic kit for brain injury, comprising: reverse transcription primers and detection primers of microRNA markers; said kit can detect the content of microRNA marker miR-9 in cerebrospinal fluid; said reverse transcription primers are:

[0055] The reverse transcription primer sequence of miR-9 is shown in SEQ ID NO:1; The detection primer is: the forward primer sequence of miR-9 is shown in SEQ ID NO:5; The reverse primer sequence of miR-9 is Shown in SEQ ID NO:6.

Embodiment 2

[0057] A diagnostic kit for brain injury, comprising: reverse transcription primers and detection primers of microRNA markers; the kit can detect the content of microRNA marker miR-124 in cerebrospinal fluid; the reverse transcription primer sequence of miR-124 is Shown in SEQ ID NO: 2; the detection primers are: the forward primer sequence of miR-124 is shown in SEQ ID NO: 7; the reverse primer sequence of miR-124 is shown in SEQ ID NO: 8.

Embodiment 3

[0059] A diagnostic kit for brain injury, comprising: reverse transcription primers and detection primers of microRNA markers; the kit can detect the content of microRNA marker miR-128a in cerebrospinal fluid; the reverse transcription primer sequence of miR-128a is Shown in SEQ ID NO:3; the detection primers are: the forward primer sequence of miR-128a is shown in SEQ ID NO:9; the reverse primer sequence of miR-128a is shown in SEQ ID NO:10.

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Abstract

The invention discloses a miRNA marker, a kit and an application for detecting brain injury. The marker is any one or more of;miR-9, miR-124, miR-128a and miR-128b, the kit is used for detecting the content of microRNA markers in cerebrospinal fluid; the marker and the kit can be used for auxiliary diagnosis of brain injury, and are easy to detect, accurate in quantity, good in sensitivity and specificity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a miRNA marker, kit and application for detecting brain damage. Background technique [0002] Traumatic brain injury (Traumatic brain injury, TBI) is one of the most basic problems in neurosurgery, and it is also a global public health problem with a high fatality rate and high disability rate. The worldwide incidence rate is about 0.15% to 0.45% per year, usually due to traffic accidents, high-altitude falls, extreme sports, etc., resulting in violent impacts on the head, causing transient amnesia, motor and cognitive dysfunction. If it is not diagnosed and treated in time, it will eventually lead to irreversible neurological damage. [0003] At present, the severity and postoperative recovery of traumatic brain injury are mostly evaluated clinically through imaging observation and behavioral scoring, which lacks sufficient accuracy and sensitivity. Contents of the invention [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156C12Q2600/178
Inventor 魏亮
Owner 成都仕康美生物科技有限公司
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