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Cervinae activin A protein and preparation method and application thereof

A technology of activin and protein A, which is applied in the field of molecular biology, can solve the problems of time-space specific deer subfamily fecundity disorder, and there is no deer subfamily ACTA protein and cDNA sequence, etc., to achieve the effect of increasing research and application prospects

Active Publication Date: 2019-02-01
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there are still few domestic studies on ACTA in the subfamily Cervidae, and there is no report on ACTA protein and cDNA sequences in the subfamily Cervidae. and the research on the fecundity of deer subfamily will cause obstacles

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  • Cervinae activin A protein and preparation method and application thereof
  • Cervinae activin A protein and preparation method and application thereof
  • Cervinae activin A protein and preparation method and application thereof

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Embodiment

[0053] 1 Materials and methods

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Abstract

The invention relates to the field of molecular biology and particularly relates to a cervinae activin A protein and a preparation method and application thereof. The activin A protein is composed oftwo cervinae activin beta A subunits isolated from cervinae animals. The study on the activin A protein is still relatively rare in the cervinae. The preparation method acquires the isolated gene sequence, improves its high-purity protein expression method, provides research basis and novel ideas for related research and greatly increases the research and application prospect of the activin A protein.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a deer subfamily activin A protein and its preparation and application. Background technique [0002] Activin (Actvin, ACT), also known as activin, is a glycoprotein hormone firstly isolated and purified from porcine follicular fluid by Vale et al. and Ling et al. It is named after the inhibition of pituitary cell secretion of follicle stimulating hormone (Follicle Stimulating Hormone, FSH), which belongs to the transforming growth factor β superfamily member. Activin is mainly synthesized and secreted by ovarian granulosa cells and placenta. Because it has the biological activity of specifically promoting the secretion and synthesis of follicle stimulating hormone by pituitary cells, it is named activin. [0003] Both ACT and INH are dimers composed of two subunits. ACT is composed of β subunits, including homodimer ACTA (βA-βA) and ACTB (βB-βB) or heterodimer ACTAB (βA-βB). ...

Claims

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Application Information

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IPC IPC(8): C07K14/575C12N15/16C12N15/85C12N5/10A61K38/22A61P43/00A61P37/02A61P25/00A61P19/08A61P5/28A61P5/30A61P15/08
CPCA61K38/00A61P5/28A61P5/30A61P15/08A61P19/08A61P25/00A61P37/02A61P43/00C07K14/575C12N15/85
Inventor 许保增张宇飞王丽英曹满媛赵伟刚杨镒峰魏海军常彤
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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