39 results about "Associated Study" patented technology

The invention discloses a method for screening disease-related proteins based on a complex network. The method comprises the following steps: 1) acquiring a seed gene related to a target disease; 2) based on a protein-protein interaction database, constructing a protein interaction network taking the seed gene as a core; 3) extracting characteristic data of the protein in the protein interaction network; 4) taking the characteristic data of the protein as training data, and training by adopting a machine learning algorithm to obtain a PU classifier; and 5) predicting the protein related to thetarget disease in the protein interaction network according to the PU classifier. The method can quickly and efficiently identify protein related to a disease, and is helpful for biomedical experts to carry out experimental verification or related researchers to carry out work.

The invention relates to application of GJB2 gene p.V37I mutation to the preparation of products for diagnosing late-onset deafness. The GJB2 gene p.V37I mutation is p.V37I exclusive mutation. The invention also provides a GJB2 gene p.V37I mutation diagnostic kit for diagnosing the late-onset deafness and application of the kit. The GJB2 gene p.V37I mutation diagnostic kit has the advantages that: a common molecular identifier, namely the GJB2 gene p.V37I exclusive mutation related to the late-onset deafness highly is discovered at home and abroad for the first time; a molecular identifier-based gene screening method and the preferential application range of the molecular identifier in the screening of neonates are determined; and the alarm time of the late-onset deafness caused by the GJB2 gene p.V37I exclusive mutation is advanced to a neonate period, and the GJB2 gene p.V37I mutation diagnostic kit is widely applied to the early detection, intervention and rehabilitation of genetic susceptible late-onset deafness, the screening and diagnosis of deafness genes, late-onset deafness hearing-gene combined screening and other relevant study fields.

The invention belongs to the technical field of biotechnology and diagnostic detection, and discloses a PCR detection primer for Riemerella anatipestifer virulent phage and a kit thereof, wherein the primers are shown in SEQ ID NO: 5 and SEQ ID NO: 6, The Riemerella anatipestifer virulent phage can be quickly identified simply by using a pair of specific primers to amplify the sample to be tested by PCR and then electrophoresis. At present, there is no report on the identification of Riemerella anatipestifer virulent phage based on molecular biology methods. This study can fill the gap in related research fields.

The invention discloses a reference gene developed based on a transcriptome sequence of miscanthus sinensis and application thereof. The three reference gene sequences of the invention come from the transcriptome sequence of miscanthus sinensis, and have the advantages of good specificity and high stability compared with the previous reference genes commonly used in other species. At the same time, the expression analysis of SOD and CAT genes under drought stress of miscanthus sinensis proves that the developed three reference genes have better calibration capability than other general reference genes, and provides an effective reference gene calibration tool for the analysis, screening and validation of the gene expression of miscanthus sinensis in the future. In addition, the newly developed reference gene enriches the available number of plant reference genes, as well as is applied to the related research on other andropogoneae and miscanthus plants. The reference gene provided by the invention is suitable for popularization and application in the field of reference genes.

The invention discloses a method for constructing a retinitis pigmentosa disease model, an application and a breeding method, and relates to the technical field of gene editing. The method disclosed in the present invention modifies the Mettl14 gene of the target animal so that the target animal exhibits typical characteristics of the retinitis pigmentosa disease, and can be used as a model of the retinitis pigmentosa disease. The method disclosed in the invention can construct a new disease model with the characteristics of retinitis pigmentosa disease. The disease model can be used for related studies on the pathogenesis and mechanism of retinitis pigmentosa disease, and can also be used for drug screening related to retinitis pigmentosa disease, and has broad application prospects.

The present invention amplifies the nucleoprotein gene N of SVCV through molecular biology techniques, and constructs recombinant plasmid pMD TM 18‑T‑SVCV‑N, using this plasmid as a template to establish an absolute quantitative SVCV copy number method based on the TaqMan probe method qPCR as a standard curve. At the same time, the protein concentration of SVCV was measured by Bradford method, and the correlation with the copy number of SVCV measured by the qPCR method was established. For the first time, a method for directly measuring protein concentration to determine the copy number of SVCV was established. This method can realize the simple and low-cost determination of the copy number of SVCV, and at the same time has important reference significance for the quantification of other viruses, and has important value in the related research of SVCV and other viruses.

The invention belongs to the field of biotechnology, particularly discloses a primer group and method for specific amplification of Cricetulus and Lasiopodomys Vkorc1 gene coding sequence region. Theprimer group includes 3 pairs of primers for respectively amplifying three exons of Vkorc1 genes, the primer sequence for amplifying the first exon is shown in SEQ ID NO.1-2, the primer sequence for amplifying the second exon is shown in SEQ ID NO.3-4, and the primer sequence for amplifying the third exon is shown in SEQ ID NO.5-6. The invention provides the amplification method based on the primer group through optimizing the amplification condition, the Cricetulus and Lasiopodomys Vkorc1 genes are amplified, and a good foundation is laid for previous related research on the Cricetulus and Lasiopodomys Vkorc1 genes and resistance to drugs.

The invention discloses a method for screening disease-related proteins based on a complex network. The method is as follows: 1) Obtain the seed gene related to the target disease; 3) extracting the feature data of the protein in the protein interaction network; 4) using the feature data of the protein as training data, and using a machine learning algorithm to train a PU classifier; 5) predicting the PU classifier according to the PU classifier Proteins in the protein interaction network associated with the target disease. The method of the invention can quickly and efficiently identify proteins related to diseases, and is helpful for biomedical experts to carry out experimental verification or relevant researchers to carry out work.