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Automatic typing method of MAOAmu-VNTR fluorescent labels

A genotyping method and fluorescent labeling technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of accuracy relying on individual experience, long electrophoresis time, manual interpretation of genotyping, etc. The effect of application prospect, short detection time and high degree of automation

Inactive Publication Date: 2016-12-21
KUNMING MEDICAL UNIVERSITY
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Problems solved by technology

However, most of the current research reports on MAOAμ-VNTR polymorphisms use PCR amplification combined with agarose gel electrophoresis. Genotyping relies on manual interpretation, and the accuracy depends on individual experience, so there are certain errors; and the electrophoresis takes a long time

Method used

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  • Automatic typing method of MAOAmu-VNTR fluorescent labels
  • Automatic typing method of MAOAmu-VNTR fluorescent labels

Examples

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Embodiment example 1

[0026] Implementation Case 1, the GeneMapper IDv3.2 software for VNTR typing includes creating a new Panel, creating a new GeneMapper Manager, creating a new Size Standards and clicking to open the GeneMapper IDv3.2 software. Its usage method of described newly-built Panel is to click Tools to select Panel Manager in the drop-down menu, click New Kit in the File drop-down menu of the window that jumps out, edit, analyze method name such as MAOA, select MAOA, create a new New Marker and edit segment name, size area. The method of using the newly-created GeneMapper Manager is to click Tools to select GeneMapper Manager in the drop-down menu, click analysis method in the window that pops up, click New and name it such as MAOA, select the edited Panel, and click OK. The method of using the newly-created Size Standards is to click Tools to select GeneMapper Manager in the drop-down menu, click Size Standards in the window that pops up, click New and name it such as MAOA-liz, write ...

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Abstract

The invention relates to the field of fluorescently-labeled primer typing, in particular to an automatic typing method of MAOAmu-VNTR fluorescent labels. The method comprises the steps of sample and DNA extraction, PCR amplification, capillary electrophoresis conducted through a 3130 type genetic analyzer and VNTR typing conduced through GeneMapper IDv3.2 software. The automatic typing method of the MAOAmu-VNTR fluorescent labels has the advantages that the typing efficiency of the built automatic typing method of the MAOAmu-VNTR polymorphic fluorescent labels is high, and by means of full-automatic capillary electrophoresis, MAOAmu-VNTR genotyping can be conducted more accurately; the advantages of being simple in sample treatment, high in degree of automation, short in detection time and the like are achieved, the result is accurate and reliable, operation is easy and convenient, and a good application prospect in related research for MAOAmu-VNTR polymorphism is achieved.

Description

technical field [0001] The invention relates to the field of fluorescent marker primer typing, in particular to a MAOAμ-VNTR fluorescent marker automatic typing method. Background technique [0002] At present, domestic and foreign scholars' research on MAOAμ-VNTR polymorphism is mostly focused on alcohol dependence, schizophrenia, depression, and violent aggressive behavior. It provides a theoretical basis for control, risk prediction, and drug design for alcohol dependence and violent aggression, and has important socioeconomic significance. However, most of the current research reports on MAOAμ-VNTR polymorphisms use PCR amplification combined with agarose gel electrophoresis. Genotyping relies on manual interpretation, and the accuracy depends on individual experience, so there are certain errors; and the electrophoresis takes a long time. [0003] Therefore, it is necessary to propose an automatic typing method for MAOAμ-VNTR fluorescent markers to solve the above prob...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2565/125C12Q2563/107C12Q2537/165
Inventor 聂胜洁聂爱婷胡利平顾涛张秀峰张素仙胡万芹杜雷饶旼王美芬
Owner KUNMING MEDICAL UNIVERSITY
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