Neural stem cell induction differentiation medium and neural stem cell induction differentiation method

A technology for neural stem cell induction and differentiation, applied in the field of neural stem cell induction differentiation medium and neural stem cell induction differentiation, can solve the problems of instability, long cycle, high differentiation cost, etc., and achieve the effect of clear ingredients, simple ingredients, and short differentiation time

Pending Publication Date: 2019-02-01
BEIJING CELLAPY BIOTECH
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Problems solved by technology

[0012] The present invention aims to provide a neural stem cell induction differentiation medium and a method for neural stem cell induction differentiation to solve the technical problems of high cost, long period and instability of neural stem cell induction differentiation in the prior art

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  • Neural stem cell induction differentiation medium and neural stem cell induction differentiation method
  • Neural stem cell induction differentiation medium and neural stem cell induction differentiation method
  • Neural stem cell induction differentiation medium and neural stem cell induction differentiation method

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Embodiment Construction

[0029] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0030] Aiming at the technical problems existing in the induction and differentiation process of neural stem cells in the prior art, the present invention provides an induction medium suitable for culture of feeder-free cells and fully defined hiPSCs directed differentiation into neural stem cells. The method of using the medium Simple and clear process.

[0031] According to a typical embodiment of the present invention, a neural stem cell differentiation medium is provided. The neural stem cell differentiation medium includes basal medium and additives; wherein, the basal medium is DMEM / F12 medium; the additives include 1-5 mM, 2-8 μM of GSK-3 inhibitor, 2-10 μM of BMP inhibitor, ALK inhibi...

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Abstract

The invention discloses a neural stem cell induction differentiation medium and a neural stem cell induction differentiation method. Specifically, the neural stem cell induction differentiation mediumcomprises a basic medium and additives. The basic medium is a DMEM/F12 medium. The additives include 1-5mM glutamine, a 2-8microM GSK-3 inhibitor, a 2-10microM BMP inhibitor, a 2-10microM ALK inhibitor, 0.5%-2% of a cell culture additive and 0.5%-2% of a double-antibody. The neural stem cell induction differentiation medium provided by the invention has the characteristics of clear and simple components, high differentiation efficiency, short differentiation time, no animal-derived component, no need for animal-derived cells to serve as the feeder layer during differentiation, no need to formEB, and no need for repeated replacement of the culture solution components in differentiation, and can acquire a large number of high purity neural stem cells within a short time.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a neural stem cell induction differentiation medium and a neural stem cell induction differentiation method. Background technique [0002] In 2006 and 2007, Japanese scholar Shinya Yamanaka successfully introduced four transcription factors (Oct4, Sox2, Klf4 and c-Myc) into mice respectively (Takahashi and Yamanaka., 2006). and human (Takahashi et al., 2007) skin fibroblasts were reprogrammed into induced pluripotent stem cells (Induced Pluripotent Stem Cells, iPSCs), and thus won the 2012 Nobel Prize in Medicine and Physiology. hiPS cells (human induced pluripotent stem cells) have all the differentiation capabilities of hES cells (human embryonic stem cells) and have no ethical issues. They will completely replace hES cells in the near future and become the most important cell source for regenerative medicine. [0003] Neuroscience research includes the whole process of it...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2500/32
Inventor 鲁文静兰峰
Owner BEIJING CELLAPY BIOTECH
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