Unlock instant, AI-driven research and patent intelligence for your innovation.

RFF1 cell

A technology of cellular and cellular immunity, applied in the biological field, can solve the problems of high killing rate and inability to balance the attack and defense of immune cells, achieving the effect of high safety and improving adaptability

Active Publication Date: 2019-02-01
BEIJING DCTY BIOTECH CO LTD
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to provide a RFF1 cell used for cellular immunotherapy, combined with polypeptide shock and in vitro co-cultivation, transforms ordinary T cells into super T cells capable of both attack and defense, and solves the problem that immune cells cannot balance attack and defense in cellular immunotherapy problem, good accuracy and high kill rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • RFF1 cell
  • RFF1 cell
  • RFF1 cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0048] Tumor cell immunotherapy is an emerging tumor treatment modality. In terms of tumor cell immunotherapy, the existing LAK cells, DC cells, CIK cells, and DC-CIK cells have basically been proven to be ineffective. The embodiment of the present invention provides a new T cell for cellular immunotherapy.

[0049] The embodiment of the present invention provides a kind of RFF1 cell for cellular immunotherapy, wherein the preparation method of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides an RFF1 cell, and belongs to the field of biotechnologies. The RFF1 cell used for cellular immunotherapy, provided by the invention, is a defensive and offensive super T cell, and has a high accuracy and a high killing rate. A method for preparing the RFF1 cell comprises the following steps: a PBMC is loaded with a tumor mutation-causing polypeptide, and the polypeptide loaded PBMC is subjected to polypeptide impact; amplification culture is carried out after the impact to obtain an FF cell; the tumor mutation-causing polypeptide is used as an antigen to directly stimulate the FF cell in order to screen an accurate polypeptide; an immunosuppressive signal molecule on the PBMC is knocked out; and the PBMC is loaded on the accurate polypeptide, and the loaded PBMC andthe immunosuppressive signal molecule knockout PBMC are mixed and co-cultured, and are impacted with the accurate polypeptide multiple times in the co-culturing process in order to obtain the RFF1 cell. The RFF1 cell can be used in a cellular immunotherapy technique.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a RFF1 cell used for cellular immunotherapy and a preparation method thereof. Background technique [0002] Tumor cell immunotherapy is an emerging tumor treatment model. It collects immune cells from the patient, then cultures and expands them in vitro, and then infuses them back into the patient to stimulate and enhance the body's autoimmune function to treat tumors. Tumor cell immunotherapy is the fourth tumor treatment method after surgery, radiotherapy and chemotherapy. [0003] Normal or bioengineered human cells are transplanted or infused into patients, and the newly infused cells can replace damaged cells, or have stronger immune killing functions, so as to achieve the purpose of treating diseases. [0004] The human cells transformed by bioengineering are transformed in the process of culturing in vitro by special methods, which can effectively kill tumor cells in patients...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/90C12N9/22A61P35/00
CPCA61P35/00C07K14/7051C12N5/0636C12N9/22C12N15/907C12N2510/00C12N2800/80C12N2810/10
Inventor 焦顺昌张嵘周子珊解佳森王海燕李营营
Owner BEIJING DCTY BIOTECH CO LTD