Heat-stable gene VIII type Newcastle disease attenuated strain exogenous gene expression vector

Abstract

The invention relates to the field of veterinary virus molecular biology, and discloses a heat-stable gene VIII type Newcastle disease attenuated strain exogenous gene expression vector, which is Newcastle disease virus NDV-rAHR09-GFP-1, and has the preservation number of CCTCC NO:V201844. According to the present invention, the study results show that the NDV-rAHR09-GFP-1 has good thermal stability under natural conditions, can maintain hemagglutination activity and infection ability after being treated for 90 min at a temperature of 56 DEG C, and can stably express GFP gene.

Description

technical field [0001] The invention belongs to veterinary virus molecular biology technology, in particular to a thermostable exogenous gene expression carrier of gene VIII attenuated Newcastle disease strain. Background technique [0002] Newcastle disease virus (Newcastle disease virus, NDV) belongs to the member of Paramyxoviridae, Paramyxovirinae, Avulavirus (Avulavirus) [Mayo, M.A.2002.Virus taxonomy-Houston 2002.ArchVirol 147:1071-1076 .]. NDV has an envelope, and its genome is a single-strand, negative-strand, non-segmented RNA virus. The genome structure pattern is: 3′-NP-P-M-F-HN-L-5′, which encodes six structural proteins in sequence: nucleocapsid Protein (NP), phosphoprotein (P), matrix protein (M), fusion protein (F), hemagglutinin-neuraminidase protein (HN) and macromolecular egg; (L) is nucleoprotein (NP), P, M, F, HN and L proteins. According to the virulence to chickens, NDV strains can be divided into three types: strong virulence (Velogenic), medium vir...

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Problems solved by technology

However, as an attenuated live vaccine, cold chain maintenance is required during storage and transportation. General

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