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Extraction method for biological cells on material evidence object

A technology of biological cells and extraction methods, applied in the field of biological cell extraction from physical evidence objects, can solve the problems of difficult extraction, low degree of nudity, and high concealment, and achieve the effect of improving the detection rate of cases.

Active Publication Date: 2019-02-05
SUZHOU XIAOSONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the small size of biological cell particles, especially the biological cells that have been shelved for a long time and have been fully dried, the contact between the cell body and the surface of the evidence object is high, and the contact attraction and collagen adhesion between the cell and the object surface can be much greater than that of the wipe. In addition, the cells still have the characteristics of high concealment, low exposure, and difficult extraction. It is not easy to fully collect the biological cells left on the criminal evidence by using traditional extraction methods.

Method used

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  • Extraction method for biological cells on material evidence object
  • Extraction method for biological cells on material evidence object
  • Extraction method for biological cells on material evidence object

Examples

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Effect test

Embodiment 1

[0032] Embodiment 1: the extraction method of the biological cell on the stone that deposits 30 days, comprises the steps:

[0033] (1) The biological traces on the stone were visualized by the bio-tracking fluorescent agent of Suzhou Xiaosong Technology Development Co., Ltd.

[0034] (2) Use a nylon filament brush to reciprocate up and down on the position where biological traces appear on the stone.

[0035] (3) Use a cell adsorber as disclosed in CN201721104962.7 to adsorb the debris obtained by scraping in step (1).

[0036] (4) Submit the material collected in step (3) for DNA identification, see the DNA identification results figure 1 ,From figure 1 It can be seen that the DNA was detected successfully and effectively.

Embodiment 2

[0037] Embodiment 2: the extraction method of the biological cell on the brick that deposits 30 days, comprises the steps:

[0038] (1) The biological traces on the bricks were visualized by using the bio-tracking fluorescent agent of Suzhou Xiaosong Technology Development Co., Ltd.

[0039] (2) Use a brush with static electricity to scrape in parallel reciprocatingly on the position where biological traces appear on the brick.

[0040] (3) Use a polyester film with a rubber surface with an electrostatic field to carry out electrostatic adsorption and extraction of the biological traces. The voltage of the additional electrostatic field is 2000V, a negative electric field is added to the brick, and a positive electric field is added to the polyester film. The biological trace positions are in contact.

[0041] (4) Submit the material collected in step (3) for DNA identification, see the DNA identification results figure 2 ,From figure 2 It can be seen that the DNA was det...

Embodiment 3

[0042] Embodiment 3: the method for extracting biological cells on the cement blocks stored for 30 days, comprising the steps of:

[0043] (1) The biological traces on the cement block were visualized by using the bio-tracking fluorescent agent of Suzhou Xiaosong Technology Development Co., Ltd.

[0044] (2) Rotate and scrape the cement block with a brush with nylon filaments.

[0045] (3) Under the action of an electrostatic field, the polyester film with a rubber surface is used to absorb and extract the position of the biological trace. The voltage of the additional electrostatic field is 2000V, a negative electric field is added to the brick, and a positive electric field is added to the polyester film. The biological trace positions are in contact.

[0046] (4) Submit the material collected in step (3) for DNA identification, see the DNA identification results image 3 ,From image 3 It can be seen that the DNA was detected successfully and effectively.

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Abstract

The invention relates to an extraction method for biological cells on a material evidence object. The extraction method comprises the following steps of (1) adopting a method of mechanical scratchingto perform scratching on a biological trace position of the material evidence object; and (2) collecting chippings scratched in the step (1) through a vacuum adsorption method, an electrostatic adsorption method or a viscous taking method. The extraction method provided by the invention can fully and effectively perform extraction of the cells so as to provide guarantee to subsequent DNA identification and increase the detection rate.

Description

technical field [0001] The invention relates to a method for extracting biological cells on a physical evidence object. Background technique [0002] In the field of criminal evidence investigation, it is necessary to discover and obtain criminal evidence left by suspects, including fingerprint evidence, palm print evidence, and trace evidence that can reflect DNA characteristics, so as to build a criminal evidence chain and lay a legal evidence foundation for punishing crimes . [0003] At present, Xiaosong Technology has been able to quickly and accurately lock the location of biological traces through laser irradiation fluorescence display technology. However, after the location of the biological trace is locked, the biological cells at the location still need to be fully and effectively extracted in order to provide a strong guarantee for subsequent DNA identification. However, at present, the phenomenon of "the position of the trace can be seen, but the DNA cannot be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
CPCC12N5/00C12N2509/10
Inventor 胡晓松
Owner SUZHOU XIAOSONG TECH DEV
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