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Biological activity conditioning agent for improving greenhouse soil salinization, and application thereof

A biological activity and salinization technology, applied in the field of soil conditioners, can solve the problems of not being able to effectively improve soil biological properties, soil salinization, and reduce soil salt content, so as to improve microbial flora and biological activity , Improve the performance of soil water and fertilizer retention, and balance the effect of soil nutrients

Active Publication Date: 2019-02-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These soil conditioners used to improve soil salinization are mostly non-biological conditioners, mainly natural minerals, synthetic polymers and artificial extracts, which cannot effectively improve soil biological properties and have limitations.
Zhang Furong and Yang Qian's research shows that biochar can reduce soil salinity content and improve soil microorganisms, but the single application of biochar has short timeliness and in some cases has certain side effects on yield and certain soil properties
CN107382453A discloses a soil sterilization regeneration anti-continuous cropping obstacle composition, the patent conditioner is prepared from yeast, bacillus, Trichoderma, Glioma, organic catalysts, minerals, surfactants, organic biomass , the conditioner can alleviate soil salinization and change soil microorganisms, but because it contains natural minerals and surfactants, it may aggravate soil salinization in the later stage
However, soil conditioners currently used to improve soil biological properties cannot improve soil salinization

Method used

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  • Biological activity conditioning agent for improving greenhouse soil salinization, and application thereof
  • Biological activity conditioning agent for improving greenhouse soil salinization, and application thereof
  • Biological activity conditioning agent for improving greenhouse soil salinization, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Trichoderma viridans TV41 bacterial acid and salt tolerance determination

[0048] Trichoderma viride TV41 was inoculated into potato dextrose agar medium with pH 3, 4, 5, 6, 7, 8, 9, 10, 11 in sequence, and cultured at 28°C for 3 days. The photos of the petri dish are as follows: figure 1 Shown; detect the total number of spores on the medium, such as image 3 As shown, it can be seen that TV41 Trichoderma sporulation is still relatively large under pH4-5 acidic conditions, up to 2.9 × 10 9 More than CFU.

[0049] Trichoderma viride TV41 bacterium is inoculated to the potato dextrose of 0%, 0.6%, 1.2%, 1.8%, 2.4%, 3%, 3.6%, 4.2%, 4.8% in salt concentration (salinity is adjusted with NaCl reagent) successively In the agar medium, culture at 28°C for 2 days, the photo of the petri dish is as follows figure 2 Shown; detect the total number of spores on the culture medium, the result of spore quantity is as follows Figure 4 As shown, it can be seen that the...

Embodiment 2

[0050] Example 2 Bacillus amyloliquefaciens BS211 bacterial acid and salt tolerance determination

[0051] Bacillus amyloliquefaciens BS211 was inoculated into beef extract peptone medium with different pH values ​​and cultured at 30°C for 3 days to detect the number of viable bacteria. The results were as follows: Figure 5 As shown, BS211 bacillus cultured under acidic conditions of pH4-5 still had a large number of viable bacteria up to 5.1×10 8 More than CFU.

[0052] In addition, BS211 bacteria were inoculated into beef extract peptone medium with different salt concentrations (the salt concentration was adjusted with NaCl reagent) and cultured at 30°C for 3 days to detect the number of viable bacteria. The test results were as follows: Figure 6 As shown, it can be seen that the number of viable bacteria cultured by BS211 bacillus at 3% salt concentration is still high, which is basically consistent with the number of viable bacteria at 0% salt concentration.

Embodiment 3

[0053] Embodiment 3 prepares Trichoderma viride TV41 bacterial agent

[0054] a) Inoculate the Trichoderma viride TV41 bacterium preserved on the slant into the potato dextrose agar medium, and produce green conidia after culturing at 28°C for 3 days; then insert the Trichoderma viride mycelium block with the conidia into the potato dextrose liquid medium In the medium, the volume of the liquid culture medium is 80m1 / 250m1, and each bottle is inoculated with 3 pieces of mycelium with a diameter of 8mm. Then culture at 28° C. on a shaker at 160 rpm for 3-4 days to obtain mycelia liquid.

[0055] b) Insert 1.5kg of sterilized bran straw solid culture medium into the cultivated 80ml Trichoderma mycelia liquid, and after fully mixing well under aseptic conditions, spread the solid culture in a shallow dish (the shallow dish passes through Soak in formalin solution overnight and dry), the thickness is 3-5cm, the bottom of the shallow plate is covered with autoclaved filter cloth, ...

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Abstract

The invention discloses a biological activity conditioning agent for improving greenhouse soil salinization. The conditioning agent is prepared from, by mass percentage, 10-20% of bio-charcoal, 0.5-1%of trichoderma viride agent, 0.5-1% of bacillus amyloliquefaciens agent and the balance of low-salt organic fertilizer. The biological activity conditioning agent has the advantages of obvious soil conditioning function on salinized greenhouse soil, capability of effectively reducing the salt content of the soil, increasing microbial activity and diversity of the soil, increasing the number of beneficial microorganisms of the soil and improving the soil fertility quality, and benefit to production and income increase.

Description

technical field [0001] The invention relates to the field of soil conditioners, in particular to a biologically active conditioner for improving soil salinization in facilities. Background technique [0002] China's protected cultivation area has reached 3.7 million hectares, and the output value has exceeded 980 billion yuan. Facility cultivation has the characteristics of high multiple cropping index and high planting density, so a large amount of chemical fertilizers and high-nutrient organic materials are used for a long time. The high temperature, high humidity, and semi-enclosed environment of the facility lead to a large amount of nutrients and other salts that have not been absorbed by the crops remaining in the soil of the plow layer, making the secondary salinization of the soil serious. The living environment of soil microorganisms is destroyed, the growth of beneficial microorganisms is inhibited, and harmful microorganisms are easy to reproduce, causing microbi...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05D9/00C05G3/00C05G3/80C05F5/00C05F5/002C05F5/006C05F11/00C05F11/08
Inventor 王光飞马艳郭德杰王秋君徐烨红艾玉春
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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