Identification method of pesticide residues in cucumber skin based on near-infrared spectroscopy

A technology of near-infrared spectroscopy and pesticide residues, which is applied in the field of identification of pesticide residues on cucumber skin, can solve the problems of low detection accuracy and the inability to carry out qualitative classification and identification of pesticide residues, and achieve the effect of simple detection device, low cost and high sensitivity

Inactive Publication Date: 2021-07-16
LESHAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The existing technology represented by the above-mentioned patent documents can realize the detection of pesticides, but it cannot qualitatively classify and identify the pesticide residues with different concentration gradients on the cucumber epidermis, and the detection accuracy is low.

Method used

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  • Identification method of pesticide residues in cucumber skin based on near-infrared spectroscopy
  • Identification method of pesticide residues in cucumber skin based on near-infrared spectroscopy
  • Identification method of pesticide residues in cucumber skin based on near-infrared spectroscopy

Examples

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Effect test

Embodiment 1

[0042] A method for identifying pesticide residues on cucumber epidermis based on near-infrared spectroscopy, comprising the following steps:

[0043] a. Sample preparation

[0044] Three cucumbers were selected, one of which was not sprayed with pesticides, as a sample without pesticides; the liquid pesticide aviacidamiprid and tap water were selected to form a pesticide aqueous solution of 1:1000 and 1:100, and the other two cucumbers were treated by spraying. Cucumbers were sprayed; cucumbers sprayed with a pesticide concentration of 1:1000 were used as samples containing mild pesticide residues, and cucumbers sprayed with a pesticide concentration of 1:100 were used as samples containing heavy pesticide residues;

[0045] b. Spectral data collection

[0046] After 24 hours of spraying pesticides, pick three cucumber samples for spectral data collection, and take 4mm of interrupted epidermis from each cucumber 2 Make 80 specimens, collect their spectral data respectively,...

Embodiment 2

[0060] A method for identifying pesticide residues on cucumber epidermis based on near-infrared spectroscopy, comprising the following steps:

[0061] a. Sample preparation

[0062] Three cucumbers were selected, one of which was not sprayed with pesticides, as a sample without pesticides; the liquid pesticide aviacidamiprid and tap water were selected to form a pesticide aqueous solution of 1:1000 and 1:100, and the other two cucumbers were treated by spraying. Cucumbers were sprayed; cucumbers sprayed with a pesticide concentration of 1:1000 were used as samples containing mild pesticide residues, and cucumbers sprayed with a pesticide concentration of 1:100 were used as samples containing heavy pesticide residues;

[0063] b. Spectral data collection

[0064] After 24 hours of spraying pesticides, pick three cucumber samples for spectral data collection, and take 4mm of interrupted epidermis from each cucumber 2 Make 80 specimens, collect their spectral data respectively,...

Embodiment 3

[0079] A method for identifying pesticide residues on cucumber epidermis based on near-infrared spectroscopy, comprising the following steps:

[0080] a. Sample preparation

[0081] Three cucumbers were selected, one of which was not sprayed with pesticides, as a sample without pesticides; the liquid pesticide aviacidamiprid and tap water were selected to form a pesticide aqueous solution of 1:1000 and 1:100, and the other two cucumbers were treated by spraying. Cucumbers were sprayed; cucumbers sprayed with a pesticide concentration of 1:1000 were used as samples containing mild pesticide residues, and cucumbers sprayed with a pesticide concentration of 1:100 were used as samples containing heavy pesticide residues;

[0082] b. Spectral data collection

[0083] After 24 hours of spraying pesticides, pick three cucumber samples for spectral data collection, and take 4mm of interrupted epidermis from each cucumber 2 Make 80 specimens, collect their spectral data respectively,...

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Abstract

The invention discloses a method for identifying pesticide residues on cucumber epidermis based on near-infrared spectrum analysis, which belongs to the field of pesticide residue detection, and is characterized in that it comprises the following steps: a. sample preparation; b. spectral data collection; c. principal component analysis spectrum Data dimensionality reduction; d. Support vector machine spectral data classification and identification; first step, randomly generate 150 sample data in the training set and 90 sample data in the test set; second step, perform normalization processing; third step, use Gaussian The radial basis kernel function creates a support vector machine model; the fourth step is to use the grid search and cross-validation method to obtain the optimal model of the support vector machine with the best parameter C and the best parameter g; the fifth step is to apply the support vector machine respectively The optimal model identifies the normalized training set data and predicts the normalized test set data. The invention can qualitatively classify and identify pesticide residues with different concentration gradients on the cucumber epidermis, and has high detection accuracy.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, in particular to a method for identifying pesticide residues on cucumber epidermis based on near-infrared spectrum analysis. Background technique [0002] Cucumber is an annual vine or climbing herb of Cucurbitaceae, also known as pickled cucumber and cucumber. It is favored by the public because it is rich in nutrients such as water, protein, and vitamins, and has the functions of clearing away heat, quenching thirst, diuresis, and reducing swelling. The cucumber planting cycle is long, and it is vulnerable to diseases and insect pests such as downy mildew, powdery mildew, gray mold, bacterial angular spot, and leaf blight. In order to ensure production, melon farmers often spray pesticides frequently, or spray pesticides with excessive concentrations, resulting in pesticide residues on the surface of cucumbers, which not only harms people's health, but also affects the expo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 李敏胡丹曹进蒋强
Owner LESHAN NORMAL UNIV
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