Cotton verticillium wilt resistance gene gbcyp86a1-1 and its application
A technology for verticillium wilt and cotton, which is applied to the cotton GbCYP86A1-1 gene and its application field, and can solve the problems of difficulty in treatment, affecting cotton yield and quality, and serious harm of cotton verticillium wilt.
Active Publication Date: 2021-06-18
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
However, Verticillium wilt of cotton is very harmful. Because its pathogenic fungus has the characteristics of wide distribution, serious damage, wide host range, fast transmission speed and long survival time in the soil, it is difficult to control and seriously affects the yield and quality of cotton. , known as the "cancer" of cotton
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[0061] The present invention utilizes biological information, and 10 CYP86 family genes have been identified from the genome that have been released Dielmond's cotton, and further from the sequencing terrestrial TM-1 and island cotton 3-79 The homologous gene is discovered, and the system cluster analysis is divided into three categories of A, B, and C. The expression analysis of tissue organs found that the CYP86A1 gene in cotton was specifically expressed in the roots of plants. The CYP86A1-1 gene expression level is significantly associated with disease resistance. Compared with the Test Isolated Land Cotton Wood, the gene is significantly enhanced in the anti-diseased island cotton H7124 tissue, its expression and induced expression levels are significantly increased. Based on PCR technology, the genomic sequence and full length ORF sequence of CYP86A1-1 were obtained in the island cotton H7124, which generated in the genomic A subgroup (GBCYP86A1-1A) in heterologous tetraploi...
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The invention discloses a gene GbCYP86A1‑1 resistant to cotton verticillium wilt and its application, belonging to the field of biotechnology applications. The GbCYP86A1-1 gene involved in the present invention encodes a fatty acid ω-hydroxylase. The present invention provides the genome and full-length ORF nucleotide sequence (the gene has no introns) and amino acid sequence of GbCYP86A1‑1 in the genome A subgroup and D subgroup of the allotetraploid sea island cotton H7124. The CYP86A1‑1 gene in cotton is specifically expressed in roots, and its expression is significantly upregulated by Verticillium dahliae. Compared with the susceptible upland cotton variety Junmian 1, the expression and induced expression levels of this gene were significantly enhanced in the root tissues of the disease-resistant sea-island cotton variety H7124. Through the identification of Verticillium wilt resistance, silencing the gene based on VIGS technology in sea island cotton H7124 can significantly reduce the Verticillium wilt resistance of cotton, and overexpressing the gene in Arabidopsis can significantly improve the disease resistance of transgenic Arabidopsis sex.
Description
Technical field [0001] The present invention belongs to the field of biotechnology, involving a cotton GBCYP86A1-1 gene and its application thereof, which encodes a fatty acid ω-hydroxylase. By systematically analyzing the CYP86 subfamily members in cotton pigment P450 gene familys, excavation of the genetic genes that can significantly improve cotton and Arabidopsis flavor. The CYP86A1-1 gene in cotton is specially expressed in the roots, which is protected significantly from vana. Compared with the No. 1 Military Take Cotton Variety, the gene is significantly enhanced in the H7124 tissue of the diseased island cotton variety. The genomic sequence, full length ORF sequence, and encoded amino acid sequences were obtained using PCR techniques in the island cotton H7124. The disease resistance and function of the gene was studied using biotechnology. Through vana resistance identification, VIGS technology based on the sank of the island cotton h7124 will significantly reduce the vul...
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IPC IPC(8): C12N15/53C12N9/02C12N15/82A01H5/00A01H6/60
CPCC12N9/0071C12N15/8282
Inventor 郭旺珍王桂林
Owner NANJING AGRICULTURAL UNIVERSITY