Cell cryopreservation solution and application thereof

A cryopreservation and cell technology, applied in the field of cell biology, can solve the problems of unstable batches of immunogenicity and serum contamination, and achieve the effects of good batch-to-batch stability, avoiding pollution and stable components.

Inactive Publication Date: 2019-03-08
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although DMSO is used as a cell-permeable cryoprotectant, its cytotoxicity cannot be ignored when stem cells are use

Method used

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  • Cell cryopreservation solution and application thereof
  • Cell cryopreservation solution and application thereof
  • Cell cryopreservation solution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] An embodiment of the cell cryopreservation solution of the present invention, the cell cryopreservation solution of this embodiment is composed of basic medium, glycerol, proline, tetrahydromethyl pyrimidine carboxylic acid and dextran, the basic medium, The volume ratio of glycerol and tetrahydromethyl pyrimidine carboxylic acid is basic medium: glycerol: tetrahydromethyl pyrimidine carboxylic acid=100:40:25; The volume of described basic medium and proline and dextran The mass ratio is basic medium:proline:dextran=100mL:10g:15g.

[0041] The preparation method of the cell cryopreservation solution in this example is as follows: according to the ratio described in this example, add glycerol, proline, tetrahydromethylpyrimidine carboxylic acid and dextran to the basic medium, mix well, and obtain the The above-mentioned cell freezing solution.

Embodiment 2

[0043]An embodiment of the cell cryopreservation solution of the present invention, the cell cryopreservation solution of this embodiment is composed of basic medium, glycerol, proline, tetrahydromethyl pyrimidine carboxylic acid and dextran, the basic medium, The volume ratio of glycerol and tetrahydromethyl pyrimidine carboxylic acid is basic medium: glycerol: tetrahydromethyl pyrimidine carboxylic acid=100:20:15; The volume of described basic medium and proline and dextran The mass ratio is basic medium:proline:dextran=100mL:5g:5g.

[0044] The preparation method of the cell cryopreservation solution in this example is the same as that in Example 1.

Embodiment 3

[0046] An embodiment of the cell cryopreservation solution of the present invention, the cell cryopreservation solution of this embodiment is composed of basic medium, glycerol, proline, tetrahydromethyl pyrimidine carboxylic acid and dextran, the basic medium, The volume ratio of glycerol and tetrahydromethyl pyrimidine carboxylic acid is basic medium: glycerol: tetrahydromethyl pyrimidine carboxylic acid=100:50:30; The volume of described basic medium and proline and dextran The mass ratio is basic medium:proline:dextran=100mL:15g:20g.

[0047] The preparation method of the cell cryopreservation solution in this example is the same as that in Example 1.

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Abstract

The invention relates to a cell cryopreservation solution and the application thereof, and belongs to the field of cell biotechnology. The cell cryopreservation solution comprises a minimal medium, glycerol, proline, tetra-hydro-methyl pyrimidine carboxylic acid and dextran, wherein the volume ratio of the minimal medium, the glycerol and the tetra-hydro-methyl pyrimidine carboxylic acid is 100 to20-50 to 15-30; the ratio of the volume of the minimal medium to the mass of the proline and the dextran is 100 mL to 5-15 g to 5-20 g. The cell cryopreservation solution does not contain serum or DMSO, thereby avoiding contamination of pathogenic bacteria and cytotoxicity of DMSO. The cell cryopreservation solution composed of pure chemical substances is stable in components and good in batch stability; and the viability of cells is remarkably enhanced after the cells cryopreserved with the cell cryopreservation solution are recovered.

Description

technical field [0001] The invention relates to a cell cryopreservation solution and application thereof, belonging to the technical field of cell biology. Background technique [0002] Mesenchymal stem cells are important members of the stem cell family, which can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells under specific induction conditions in vivo or in vitro. Clinical treatment of various diseases, such as nervous system diseases, kidney diseases, autoimmune diseases, malignant tumors, etc. provides a new way. Mesenchymal stem cells mainly include bone marrow mesenchymal stem cells, fat mesenchymal stem cells and umbilical cord mesenchymal stem cells. At present, bone marrow mesenchymal stem cells are the most widely studied. , the number, expansion and differentiation of bone marrow mesenchymal stem cells showed a significant downward trend, and the possibility of virus and cell in...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/0775
CPCA01N1/0221A01N1/0226C12N5/0665
Inventor 黄燕飞车七石刘少辉
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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