Gel containing MG53 protein/MG53 mutant protein, as well as preparation method thereof and application thereof

A technology of protein gel and mutants, which is applied in the medical field to achieve the effects of promoting hyperplasia, shortening the recovery period, and relieving pain

Pending Publication Date: 2019-03-08
MUDANJIANG YOUBO PHARMA CO LTD
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention aims to propose a protein gel containing MG53 protein/MG53 mutant protein, which is used to solve the stability of MG53 protein, and ensure that the protein act

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gel containing MG53 protein/MG53 mutant protein, as well as preparation method thereof and application thereof
  • Gel containing MG53 protein/MG53 mutant protein, as well as preparation method thereof and application thereof
  • Gel containing MG53 protein/MG53 mutant protein, as well as preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0052] Example 1

[0053] An MG53 protein / MG53 mutant protein-containing gel comprises the following components: gel matrix, preservatives, antioxidants, and MG53 protein / MG53 mutant protein freeze-dried powder.

[0054] The gel base includes: deionized water, sodium carboxymethyl cellulose, hydroxyethyl cellulose, glycerol acrylate / acrylic acid copolymer, SCLEROTIUM ROLFSSII gum.

[0055] The preservatives include: one or more of ethyl paraben, sodium benzoate, potassium sorbate, methylparaben, and propylparaben. The present invention is preferably: methylparaben and propylparaben. one or both.

[0056] Antioxidants include: EDTA-2Na (disodium ethylenediaminetetraacetate).

[0057] The moisturizing agent includes: one or more of sodium hyaluronate, glycerin, 1,2-pentanediol and butanediol.

[0058] The protein protective agent includes: one or more of glycerol, propylene glycol, human albumin, dextran and trehalose.

[0059] MG53 protein / MG53 mutant protein lyophilized po...

Example Embodiment

[0060] Example 2

[0061] The difference between this embodiment and the above-mentioned embodiment is that a gel containing MG53 protein / MG53 mutant protein has the following formula and ingredients: 55w / w%-66w / w% gel matrix, 3w / w%-5w / w% preservative, 5w / w%-10w / w% antioxidant, 2w / w%-3w / w% humectant, 5w / w%-7w / w% protein protectant, 20w / w%-25w / w% MG53 protein / MG53 mutant protein lyophilized powder.

[0062] The components of the gel matrix include: 45w / w%-50w / w% deionized water, sodium carboxymethylcellulose, 0.2w / w% sodium carboxymethylcellulose, 5w / w%-10w / w % Glycerin, Acrylate / Acrylic Copolymer, Hydrolyzed Sclerotinia Gum

[0063] The components of the preservative include: one or both of 1.5w / w%-3w / w% methylparaben and 1.5w / w%-3w / w% propylparaben.

[0064] The components of the antioxidant include: 5w / w%-10w / w% EDTA-2Na.

[0065] The components of the moisturizing agent include: 0.5w / w%-3w / w% sodium hyaluronate, 0.5w / w%-3w / w% glycerin, 0.5w / w%-3w / w% 1,2-pentanedi One...

Example Embodiment

[0069] Example 3

[0070] A preparation method of MG53 protein / MG53 mutant protein gel: comprising the following steps:

[0071] S1. Preparation of gel matrix:

[0072] Add deionized water to the main emulsifying pot, sprinkle hydroxyethyl cellulose powder during stirring, and stir for 10 minutes, then heat to 75°C while stirring until the solution is completely transparent; start to cool down to 45°C, and stir well The components in disodium EDTA, glycerol (and) glycerol acrylate / propylene copolymer (and) propylene glycol sodium hyaluronate are sequentially added to the main emulsification pot to ensure that one component is completely dissolved and then the next component is added; finally a gel is obtained matrix.

[0073] S2. Mix the gel matrix, preservatives, antioxidants, humectants, and protein protectants according to a certain proportion to form a first mixed solution, and sterilize the first mixed solution by autoclaving, and the conditions of autoclaving are: 121°...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a gel containing MG53 protein/MG53 mutant protein as well as a preparation method thereof and application thereof, and belongs to the technical field of medicines. The gel containing the MG53 protein/MG53 mutant protein is prepared from the following components: a gel substrate, a preservative, an anti-oxidant, a protein protector, and MG53 protein/MG53 mutant protein freeze-dried powder. According to the gel containing the MG53 protein/MG53 mutant protein and the preparation method thereof provided by the invention, the protein active ingredients of the MG53 protein/MG53 mutant protein can be kept stable under a long-term room-temperature storage condition; healing of wound surfaces such asdiabetic feet, bedsores, burns and scalds is effectively ensured; a restoration stage of a patient is shortened; and pains of the patient are effectively relieved.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a gel containing MG53 protein / MG53 mutant protein and a preparation method and application thereof. Background technique [0002] The relative molecular weight of MG53 protein is 53000, and it is one of the family members of cardiac / skeletal muscle-specific three-domain tripartite motif (TRIM) consisting of 477 amino acids. After years of research, Weisleder et al. (Weisleder et al., 2012) found that the molecular structure of MG53 protein has 3 domains, from the N-terminus to the C-terminus are the zinc finger domain, 2 B-boxes, and 1 coiled-coil structure. , Zn is also one of the components, and its carboxyl terminal contains a SPRY structure (He et al., 2012). It is specifically expressed in skeletal muscle and cardiomyocytes. [0003] Diabetic foot and bedsore are common clinical skin ulcer diseases, which bring great pain to patients. The foot is a complex target organ i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K38/17A61K9/06A61P3/10A61P17/02
CPCA61K38/1709A61K47/42A61P3/10A61P17/02A61K9/06
Inventor 梁亚龙
Owner MUDANJIANG YOUBO PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap