A microfluidic chip for the diagnosis of acute leukemia using semaphorin 4D

Abstract

The invention relates to a micro-fluidic chip for diagnosing acute leukemia by Semaphorin 4D (four-dimensional). The micro-fluidic chip comprises a chip body, wherein the chip body is provided with asample feed chamber and a valve groove, and is also provided with a transparent observing window; the chip body is formed by sequentially thermally bonding a top plate, a cover plate and a function bottom plate from top to bottom; the top plate is provided with a first sample feed hole and a valve groove, and the right side of the valve groove is provided with a transparent observing window; the cover plate is provided with a second sample feed hole, and both sides of the second sample feed hole are respectively provided with a press concave groove; the function bottom plate is provided with asample groove, a drainage groove, a filtering groove, a reaction channel, a detection groove and a waste liquid groove; a filtering column is arranged in the filtering groove, and a plurality of reaction columns are arranged in the detection groove; the walls of the drainage groove, the filtering groove, the reaction channel, the detection groove and the waste liquid groove are respectively coated with cellulose powder; the surface of each reaction column is coated with semaphorin 4D antibody magnetic beads. The micro-fluidic chip has the advantages that the self-drive type is adopted, so that the number of required instruments and equipment is reduced; the detection steps are simplified, and the precision of detection results is improved.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a microfluidic chip for diagnosing acute leukemia by using Semaphorin 4D. Background technique [0002] Leukemia is a malignant clonal disease of hematopoietic stem cells. Clonal leukemia cells proliferate and accumulate in bone marrow and other hematopoietic tissues due to mechanisms such as uncontrolled proliferation, impaired differentiation, and blocked apoptosis, and infiltrate other non-hematopoietic tissues and organs, while inhibiting normal hematopoietic function. Clinically, various degrees of anemia, bleeding, infection, fever, and liver, spleen, lymph node enlargement and bone pain can be seen. According to reports, the incidence of leukemia in various regions of my country ranks sixth among various tumors. Semaphorin 4D is a 150kD transmembrane glycoprotein composed of 862 amino acids. Its extracellular conserved Sema structure is used to recognize recept...

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Problems solved by technology

[0004] However, the existing microfluidic chips mostly use liquid pumps to drive the detection samples to flow in the microchannels of the microfluidic chips. Samples, such as blood samples, undergo primary filtration, which has a certain impact on the test results

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