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Methods and systems for optics-based measurements with selectable excitation light paths

An excitation light, optical technology, used in color/spectral property measurement, measurement device, fluorescence/phosphorescence, etc.

Active Publication Date: 2021-08-24
MOLECULAR DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Some multimodal analytical instruments utilize dedicated cartridges to enable reconfiguration

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  • Methods and systems for optics-based measurements with selectable excitation light paths
  • Methods and systems for optics-based measurements with selectable excitation light paths
  • Methods and systems for optics-based measurements with selectable excitation light paths

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[0019] As used herein, the term "analyte" generally refers to a substance to be detected or measured by optical-based techniques. Examples of analytes include, but are not limited to: proteins (including membrane-bound proteins), antigenic substances, haptens, antibodies, toxins, organic compounds, peptides, microorganisms, amino acids, nucleic acids, hormones, steroids, vitamins, drugs (including for therapeutic purposes and those administered for illicit purposes), pharmaceutical intermediates or by-products, bacteria, viral particles, and antibodies or metabolites of any of the foregoing (if applicable), and two or more of any of the foregoing The combination.

[0020] As used herein, the term "sample" generally refers to material known to contain or suspected to contain an analyte. In practicing the subject matter disclosed herein, samples can be used directly as obtained from the source or after pretreatment to alter the characteristics of the sample. The sample may be ...

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Abstract

In optical-based sample analysis, eg fluorescence-based or absorbance-based measurements, a choice is made between a first excitation light path and a second excitation light path. A first excitation optical path directs excitation light from the light source through the excitation monochromator, through the excitation filter and to the sample. A second excitation light path directs excitation light from the light source through the excitation filter and onto the sample while bypassing the excitation monochromator. The excitation light generated by the light source is transmitted along the first excitation light path or the second excitation light path according to the selection made, thereby illuminating the sample. In response, the sample produces emitted light (transmitted light in the case of absorbance measurements), which is transmitted to and measured by a photodetector.

Description

[0001] This application claims priority to US Patent Application No. 15 / 138660, filed April 26, 2016, which is incorporated herein by reference in its entirety. technical field [0002] The present invention generally relates to methods, devices and systems for optical-based measurements on samples, such as fluorescence-based or absorbance-based measurements and the like. In particular, the invention relates to wavelength selection of excitation light by selecting among different excitation light paths, one of which includes a monochromator and the other bypasses the monochromator. Background technique [0003] Various analytical instruments have been developed for performing optical-based measurements (eg, fluorescence, luminescence, and absorbance) on samples (eg, compounds, biological materials, etc.) as part of assays useful in the life sciences industry. Many analytical instruments are designed to perform only one or a few specialized measurements. On the other hand, m...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/31G01J1/42
CPCG01N21/6408G01N21/6445G01N21/645G01N2021/174G01N21/6428G01N2201/061G01N2201/063
Inventor 约瑟夫·J·阿茨勒迈克尔·卡茨林格克劳斯·瓦格纳
Owner MOLECULAR DEVICES
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