Application of anthraquinone dimer derived from Cordyceps sinensis in the preparation of drugs for the treatment of primary lung cancer
An anthraquinone dimer, primary technology, applied in the field of natural medicine, can solve the problems of no relevant reports in the literature, and achieve the effect of inhibiting growth and cloning formation ability
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[0015] Example 1 Carry out cytotoxic activity experiment on anthraquinone dimer Cicadaquinone
[0016] 1. Materials:
[0017] 1.1 Tetrazole compound (MTS):
[0018] (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, CellTiter 96 Aqueous One Solution Reagent, Promega, USA).
[0019] 1.2 Preparation of target cells: recovery and culture of human breast cancer cell line MDA-MB-435, human liver cancer cell line HepG2, human colon cancer cell line HCT116 and human lung cancer cell line H460.
[0020] a. Take out the cryopreservation tubes of human breast cancer cell line MDA-MB-435, human liver cancer cell line HepG2, human colon cancer cell line HCT116, human lung cancer cell line H460 and human normal cell MCF10A from the liquid nitrogen tank, and quickly put them into In a 37°C water bath, shake continuously to dissolve it quickly, and transfer it into a centrifuge tube aseptically;
[0021] b. Add DMEM complete culture solution to 10mL...
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