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Three kinds of lactic acid bacteria or their combination and their application in the preparation of silage

A technology of silage and lactic acid bacteria, which is applied in the application field of preparing silage, can solve the problems of lack, aerobic microbial contamination, shortened storage period, etc., and achieve the goal of increasing lactic acid content, increasing glucose level, and improving silage quality Effect

Active Publication Date: 2021-10-01
QINGHAI ACAD OF ANIMAL SCI & VETERINARY MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since most of the currently used silage processing technologies rely on the natural fermentation of the pasture’s own microorganisms, the storage period is shortened and the nutritional value of the pasture is reduced due to insufficient packing pressure, low temperature, and contamination by aerobic microorganisms. There are greater risks and costs in the application promotion process
At the same time, there is a lack of effective, self-developed and produced silage inoculants and additive brands in Qinghai. The imported foreign biological preparations are difficult to adapt to the environment in Qinghai’s high-altitude area, resulting in poor addition effects and low silage quality.

Method used

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  • Three kinds of lactic acid bacteria or their combination and their application in the preparation of silage
  • Three kinds of lactic acid bacteria or their combination and their application in the preparation of silage
  • Three kinds of lactic acid bacteria or their combination and their application in the preparation of silage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Development of high-efficiency grass bean forage green micro-storage additive bacterial agent

[0045] 1 Real-time fluorescence quantitative PCR analysis of dominant lactic acid bacteria in yak rumen

[0046] Bacterial genomic DNA from yak rumen contents was extracted according to the instructions of OMEGA Stool DNA Kit, DNA concentration and purity were measured by NanoDrop 1000 nucleic acid quantifier, and stored at -20 figure 1 .A). Using the bacterial genomic DNA of the rumen content as a template, using species-specific primers (Table 1), and using the 16S rRNA gene of Lactobacillus as an internal reference, real-time fluorescent quantitative PCR amplification analysis was performed (see Table 2 for the reaction system and conditions), and recorded Cyclic Ct value, according to 2 -△△Ct method calculates relative quantities. the result shows( figure 1 .B): Compared with the total number of Lactobacillus, the number of Lactobacillus rhamnosus in yak was...

Embodiment 2

[0093] Example 2 Application of 3 strains of lactic acid bacteria in the mixed storage pasture of whole plant corn and arrowhead pea

[0094] 1 Experimental design

[0095] Using 50% whole plant corn + 50% arrowhead pea as raw materials, add the screened single strain and the mixture of three strains to study the effect of silage (CK group (control group): do not add any bacterial agents, add the corresponding MRS liquid Culture medium; test group I: add WL10 strain, the addition amount is 3×10 5 CFU / g fresh grass; test group II: add WL11 strain, the addition amount is 3×10 5 CFU / g fresh grass; test group III: add WL8 strain, the addition amount is 3×10 5 CFU / g fresh grass; test group IV: add WL10, WL11 and WL8 strains respectively, and the addition amount is 1×10 5 CFU / g fresh grass, table 8), carry out the determination of each index respectively in 1, 3, 7, 20, 40, 60 days after silage.

[0096] Table 8 Introduction to test groups

[0097]

[0098] 2 Results and Ana...

Embodiment 3

[0123] Example 3 Application of 3 strains of lactic acid bacteria in the mixed storage of pasture grass with whole plant corn and vetch

[0124] 1 Experimental design

[0125] Using 50% whole plant corn + 50% vetch as raw materials, the test design is the same as in Example 1.

[0126] 2 Results and Analysis

[0127] (1) The effect of adding bacterial agents on the pH of pasture mixed with whole plant corn and vetch

[0128] Figure 15 As shown, on the 3rd day of silage, the silage pH of the 4 test groups was extremely significantly lower than that of the control group, and the test group I was significantly lower than the test group III; Significantly; for 40 days of silage, the silage pH of test group II was significantly lower than that of the control group and test group III, and the difference between the other groups was not significant; for 60 days of silage, the pH of silage of test group I was significantly lower than that of the control group, test group II and II...

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Abstract

The invention discloses three kinds of lactic acid bacteria or their combination and their application in the preparation of silage. Specifically, the lactic acid bacteria are: Pediococcus acidilactici YLW002, Lactobacillus casei YLW003 and rhamnose milk Bacillus (Lactobacillus rhamnosus) YLW001 was deposited in the China Center for Type Culture Collection (CCTCC) on November 09, 2018, and the preservation numbers are CCTCC NO: M2018760, M2017761, M2018759. The lactic acid bacteria can significantly reduce the pH, ammonia nitrogen and soluble sugar content of the silage grass, significantly increase the lactic acid content, and improve the quality of the grass silage. The invention also discloses the silage added with the lactic acid bacteria. The silage can significantly increase the weight gain of beef cattle and mutton sheep, and at the same time can significantly increase the blood urea and glucose levels of beef cattle.

Description

technical field [0001] The invention relates to the field of animal feed, in particular to three kinds of lactic acid bacteria or combinations thereof and the application in preparing silage. Background technique [0002] Gramineae and leguminous forage are the annual forage crops with the largest planting area and the most widespread distribution in the pastoral areas of the Qinghai-Tibet Plateau. Among them, leguminous forages contain high protein and calcium and other nutrients, but legumes have more water content, and the water content of stems and leaves is quite different. When preparing hay, it is easy to lose leaves and is not easy to silage alone; while gramineous forages are the opposite. It contains more carbohydrates, and the mixed sowing of the two kinds of forages has higher and more stable yields than the single-sowed forages (usually the grass yield is increased by about 14% to 25%). It has balanced nutrition and good palatability. It can increase soil organi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K30/18A23K50/10C12R1/245C12R1/225C12R1/01
CPCA23K30/18A23K50/10C12N1/20A23V2400/125A23V2400/175
Inventor 王磊冯宇哲张晓卫吴国芳牛建章巩文
Owner QINGHAI ACAD OF ANIMAL SCI & VETERINARY MEDICINE
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