Lactobacillus mucosae with anti-influenza ability and application thereof

A technology of mucosal lactobacillus and influenza, applied in the field of microbiology, can solve problems such as obvious side effects

Active Publication Date: 2019-03-19
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Therefore, it may be possible to start with gut microbes and try to find new drugs or methods for the prevention a...

Method used

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  • Lactobacillus mucosae with anti-influenza ability and application thereof
  • Lactobacillus mucosae with anti-influenza ability and application thereof
  • Lactobacillus mucosae with anti-influenza ability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Screening and identification of mucosal lactobacilli

[0049] 1. Screening

[0050] Take human feces as the sample, after pretreatment, store the sample in about 20% glycerol in a -80°C refrigerator, take it out and thaw it, mix the sample and add 0.5mL sample to 4.5mL to contain 0.05% cysteine 0.9% normal saline for gradient dilution, select the appropriate gradient dilution and spread it on the MRS plate added with 0.05% cysteine, incubate at 37°C for 48h, pick typical colonies and streak them on the MRS plate for purification, pick A single colony was taken and transferred to liquid MRS medium (containing 0.05% cysteine) for enrichment and preserved in 30% glycerol to obtain strain CCFM1025 and strain F1.

[0051] 2. Identification

[0052] The genomes of CCFM1025 and F1 were extracted, the 16S rDNA of CCFM1025 and F1 were amplified and sequenced (Shanghai Sangon Bioengineering Co., Ltd.), and the sequences were compared in GenBank. The results showed th...

Embodiment 2

[0053] Embodiment 2: the cultivation of mucosal lactobacillus

[0054] Lactobacillus mucosae CCFM1025 was inserted into MRS solid medium (containing 0.05% cysteine) and cultured at 37°C for 48 hours, and its colonies were observed under a microscope, and the colonies were found to be Round and rough, transparent, and its bacteria are short rod-shaped.

[0055] Lactobacillus mucosae (Lactobacillus mucosae) CCFM1025 was inserted into MRS medium (containing 0.05% cysteine) and cultured at 37°C for 48 hours, and the growth curve was made. It is a heterofermentation that produces acid and gas from glucose.

[0056] Lactobacillus mucosae (Lactobacillus mucosae) CCFM1025 was inserted into MRS medium (containing 0.05% cysteine) and cultured at 10, 15, 20, 25, 30, 35, 40, 45, and 50°C for 48 hours to observe its Growth status: It grows well at 20-35°C, and it can still grow at 45°C, but it hardly grows at 15°C or below 15°C or 50°C.

[0057] Lactobacillus mucosae (Lactobacillus muco...

Embodiment 3

[0058] Example 3: Effects of mucosal lactobacilli on the body weight of influenza mice

[0059] Take 40 healthy ICR female mice with a body weight of 20-24g, and divide them into 5 groups randomly, and the 5 groups are respectively named: blank control group (Control), influenza model group (Model), and ribavirin drug treatment group (Treatment). , Lactobacillus mucosal intervention group (CCFM1025) administered intragastrically with Lactobacillus CCFM1025, Lactobacillus mucosal intervention group (F1) administered intragastrically with Lactobacillus F1, 8 rats in each group.

[0060] Two weeks before the experiment, the Lactobacillus Lactobacillus intervention group (CCFM1025) was intragastrically administered 10 times a day. 9 Lactobacillus mucous membranes CCFM1025 bacterial suspension dilution of CFU bacteria amount, Lactobacillus mucous membranes F1 intervention group (F1) gavaged the same amount of Lactobacillus mucous membranes F1 bacterial suspension dilution, and the ...

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Abstract

The invention discloses lactobacillus mucosae with anti-influenza ability and application thereof, and belongs to the technical field of microorganism and medicines. The lactobacillus mucosa has an anti-influenza effect, which is specifically embodied in: (1) significantly improving the degree of weight loss in influenza mice; (2) significantly improving blood levels in the influenza mice; (3) significantly improving the inflammation of respiratory tract infection of the influenza mice; and (4) significantly reducing the viral load in the lungs of the influenza mice (that is to say, significantly inhibiting the replication and proliferation of influenza virus in the bodies of the influenza mice). Therefore, the lactobacillus mucosae have a huge application prospect in the preparation of products for prevention and/or treatment of influenza.

Description

technical field [0001] The invention relates to a strain of mucosal lactobacillus with anti-influenza ability and application thereof, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Influenza is often a pandemic in autumn and winter, mainly caused by influenza virus. Influenza viruses can be divided into three types: influenza A virus, influenza B virus, and influenza C virus. Human influenza is mainly caused by influenza A virus and influenza B virus. Generally, animal influenza viruses do not infect humans, and human influenza viruses do not infect animals, but pigs are an exception. Pigs can be infected with both avian influenza virus and human influenza virus, but they are mainly infected with avian influenza virus. However, once they are infected with avian influenza virus, they can easily infect humans, causing human influenza pandemics, for example, 1819-1920 The "Spanish Flu" that occurred in ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/16A23L33/135C12R1/225
CPCA61K2035/115A23L33/135A61P31/16C12N1/20C12N1/205C12R2001/225
Inventor 陆文伟陈卫刘馨阳翟齐啸赵建新杭锋张灏
Owner JIANGNAN UNIV
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