Radix sophorae flavescentis residue polysaccharide synbiotics and preparation method and application thereof

A technology of synbiotics and medicinal residues, applied in the direction of bacteria, application, food science, etc. used in food preparation, can solve the problems of unseen and few synbiotics, and achieve the promotion of absorption, growth, and intestinal digestive enzyme activity. Effect

Inactive Publication Date: 2019-03-22
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that Sophora flavescens dregs contain about 10% polysaccharides. At present, there are few reports on the resource utilization of polysaccharides in

Method used

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  • Radix sophorae flavescentis residue polysaccharide synbiotics and preparation method and application thereof
  • Radix sophorae flavescentis residue polysaccharide synbiotics and preparation method and application thereof
  • Radix sophorae flavescentis residue polysaccharide synbiotics and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The domestication of embodiment 1 plant lactobacillus

[0033] Lactobacillus plantarum was inoculated in the MRS medium containing 5g / L Sophora flavescens polysaccharide, cultured at 30°C for 24h, continuously passed for 5 generations, and then inoculated into the MRS medium containing 2g / L Sophora flavescens polysaccharide. After 24 hours, 3 generations were passed continuously to obtain Lactobacillus plantarum adapted to polysaccharides from Sophora flavescens, and stored in a refrigerator at 2-8°C for future use.

Embodiment 2

[0034] Example 2 Preparation of polysaccharide synbiotics from Sophora flavescens dregs

[0035](1) Adjust the pH value of the MRS medium with 1 mol / L NaOH to make the pH value 6.5, and then sterilize under high temperature and high pressure for 20 minutes. 100mL MRS liquid culture medium is packed in 250mL Erlenmeyer flask, add 0.2g Radix Sophorae Radix Polysaccharide V) Proportionally add petroleum ether for degreasing twice, dry the sample after degreasing, add 10 times the amount of 80% ethanol, reflux in a water bath at 80°C for 2 hours, filter while hot, and wash the filter residue with hot 80% ethanol for 2 to 3 times , after drying the filter residue, add 10 times the amount of pure water and heat to reflux at 150°C, extract twice, 2 hours each time, and combine the extracts. Concentrate the water extract under reduced pressure to an appropriate volume, and slowly add absolute ethanol to make the alcohol content reach 80%, stand overnight at 4°C, centrifuge to obtain ...

Embodiment 3

[0042] Embodiment 3 Below in conjunction with feeding test, biochemical detection test and microbiology test, the present invention will be further described:

[0043] 1. Animal feeding experiments

[0044] (1) Selection, grouping and experimental design of experimental animals

[0045] ICR mice (4 weeks old, male, 18-22g) were purchased from Qinglongshan Animal Farm in Nanjing. All animals were fed under SPF grade conditions at 22°C and a 12-hour day-night cycle, and were adapted to feeding after 1 week. Randomly divided into 8 rats in each group, gavaged once a day, and continued gavage for 28 days. Drugs tested: Lactobacillus plantarum, Sophora flavescens polysaccharide (prepared according to the method in Example 1), and Sophora flavescens polysaccharide synbiotic (prepared in Example 2, hereinafter referred to as synbiotic). Comparative examples. See Table 1 for specific groups of experimental animals and dosages.

[0046] Table 1 Experimental Animal Grouping and Admi...

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Abstract

The invention discloses radix sophorae flavescentis residue polysaccharide synbiotics and a preparation method and application thereof. The radix sophorae flavescentis residue polysaccharide synbiotics is obtained by using radix sophorae flavescentis residue polysaccharide as a unique raw material through fermentation of lactobacillus plantarum. An experiment result shows that the prepared synbiotics is capable of remarkably improving a mouse visceral index and mouse intestinal tract digestive enzyme activity, keeping integrity and permeability of an intestinal tract mucous membrane, maintaining an intestinal tract barrier function, reducing levels of toxin, diamine oxidase, D-lactic acid and an inflammatory factor in a body, and through improving mouse intestinal tract bacterial diversityand flora composition structure, promoting growth of beneficial bacteria, reducing a number of harmful bacteria, regulating animal intestinal microecology, and reinforcing digestive ability and immunocompetence of an animal, and has an important application value.

Description

technical field [0001] The invention relates to polysaccharide synbiotics from Sophora flavescens dregs and a preparation method thereof, and belongs to the field of resource utilization technology of traditional Chinese medicine dregs and microecological preparations. Background technique [0002] TCM resource products are the country's TCM strategic materials and the material basis for safeguarding national health and developing the national pharmaceutical industry. The healthy development of TCM depends on the efficient utilization and sustainable development of TCM resources. With the rapid development of my country's large Chinese medicine industry and the continuous advancement of the health service industry, as well as the international market's favor for natural resource products, the production scale of traditional Chinese medicine and natural medicinal biological resource raw materials has been continuously expanded. At the same time, in the process of industrializ...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23K10/18A23K10/12
CPCA23K10/12A23K10/18A23L33/135A23V2400/169
Inventor 段金廒郭盛翁泽斌钱大玮
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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