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Disinfectant for hatching codfish in cages

A disinfectant and a technology for wheat ear fish, which is applied to the field of disinfectant for hatching wheat ear fish in cages, can solve the problems of difficulty in killing pathogenic microorganisms, inability to penetrate into the body of animals, halogenation of natural organic matter, and the like, achieving a long duration of drug effect. , improve hatching rate, increase the effect of content

Active Publication Date: 2021-03-30
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the use of disinfectants cannot selectively kill pathogenic microorganisms, the action time is short, and cannot penetrate into animals, so it is difficult to kill pathogenic microorganisms in animals, and the side effects are large, and it is easy to cause halogenation of natural organic matter, resulting in biological toxicity. and genotoxic halogenated disinfection by-products such as trihalomethanes, haloacetic acids, haloacetonitriles and other novel disinfection by-products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of the disinfectant that is used for net cage hatching earfish, comprises the steps:

[0024] a) Insert the camphor endophyte into the PDA liquid medium containing sucrose octaacetate and sucrose stearate, shake culture, sterilize after fermentation is completed, then separate the fermentation broth from the thalline, and obtain the fermentation The endophytic bacteria of Cinnamomum camphora can co-evolve with the host plant in the process of symbiosis with the host plant, and obtain some functional genes of the plant, so as to produce the same or similar metabolism as the host plant. The ability of the product, such as the ability to kill viruses, fungi and bacteria, and the use of camphor endophyte microbial fermentation to produce a large amount of active ingredients can reduce the consumption of camphor resources and further improve production efficiency; The special presence of esters and sucrose stearate can activate the silent gene in the c...

Embodiment 2

[0033] A disinfectant for hatching codfish in net cages. The disinfectant includes the following components and parts by weight: 43 parts of endophytic metabolites of camphor, 12 parts of sodium dichloroisocyanurate, 0.07 parts of catalpol, and 1.5 parts of casein. Citrate buffer was added until the pH of the disinfectant was 7. Among them, the endophyte metabolites of camphor contained 1.5% β-glucosidase; the citrate buffer contained citric acid and sodium hydrogen phosphate.

[0034] The preparation method of the above-mentioned metabolites of camphor endophytes is as follows: insert the camphor endophytes into PDA liquid medium containing sucrose octaacetate and sucrose stearate, shake at 28°C and 160r / min Cultivate for 6 days, sterilize after the fermentation is completed, then separate the fermented liquid from the bacteria, extract and concentrate the obtained fermented liquid to obtain the metabolites of camphor endophytes. The PDA liquid medium used contained 0.07wt% ...

Embodiment 3

[0037] A disinfectant for hatching codfish in net cages. The disinfectant includes the following components and parts by weight: 32 parts of endophytic metabolites of camphor, 7 parts of sodium dichloroisocyanurate, 0.05 parts of catalpol, and 1.2 parts of casein. Citrate buffer was added until the pH of the disinfectant was 5. Among them, the metabolites of the endophyte of camphor contained 1.4% β-glucosidase; the citrate buffer contained citric acid and sodium hydrogen phosphate.

[0038] The preparation method of the above-mentioned endophytic bacteria metabolites of Cinnamomum camphora is: insert the endophytic bacteria of Cinnamomum camphora into the PDA liquid medium containing sucrose octaacetate and sucrose stearate, shake at 25°C and 150r / min Cultivate for 5 days, sterilize after the fermentation is completed, then separate the fermentation liquid from the bacteria, extract and concentrate the obtained fermentation liquid to obtain the metabolites of camphor endophyt...

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Abstract

The invention provides a disinfectant for cage hatching of Pseudorasbora parva, and belongs to the technical field of aquaculture. A preparation method comprises the following steps: culturing by inoculating endophyte in camphor to a PDA liquid medium containing sucrose octaacetate and sucrose stearate, sterilizing after fermentation, extracting a fermentation broth obtained, and concentrating toobtain a camphor endophyte metabolite; adding a sodium hypochlorite solution and cyanuric acid into a reaction kettle and simultaneously introducing chlorine gas, reacting and cooling and filtering toobtain sodium dichloroisocyanurate; and adding a citrate buffer, catalpol and casein into the camphor endophyte metabolite and the sodium dichloroisocyanurate, and uniformly mixing to obtain the disinfectant. The disinfectant of the invention has a killing rate of more than 99% on viruses, fungi and bacteria, has better stability, high solubility, longer duration of drug effect, causes no secondary pollution to the cultured water body, and helps improve hatching rate of fish eggs, and has little effect on the growth and meat quality of the advanced fry of Pseudorasbora parva.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a disinfectant for hatching codfish in net cages. Background technique [0002] Pseudorasbora parva (Pseudorasbora parva), commonly known as Luohan fish, Mannenzi and stealing fish, etc., belongs to Cypriniformes (Cypriniformes), Cyprinidae (Cyprinidae), Gobioninae (Gobioninae), and wheat ear fish in taxonomy. The genus (Pseudorasbora), is a small fish that is widely distributed in various freshwater waters in East Asia. It has the characteristics of wide feeding habits, strong fecundity and strong adaptability. It has invaded almost all freshwater rivers, lakes and ponds in China. Small rotifers They feed on zooplankton, cladocera and copepods, aquatic insects and aquatic plants. In the past, people have long regarded it as wild fish for removal. With the change of breeding concept, people are now gradually realizing that the wheat ear fish has the advantages o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/13A01K61/17A01N63/30A01N43/64A01N25/30A01P1/00A01P3/00
CPCA01N25/30A01N43/64A01K61/13A01K61/17A01N63/10Y02A40/81
Inventor 谢晓泽田云方朱爱意
Owner ZHEJIANG OCEAN UNIV
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