Method for preparing ferritin-chitosan complex by utilizing transglutaminase and chitosan

A technology of transglutaminase and chitosan, which is applied in the direction of transferrin, chemical instruments and methods, animal/human protein, etc., can solve problems such as patent publications that have not been found, and achieve improved bioavailability and stability Sexuality, the effect of improving iron bioavailability

Active Publication Date: 2019-03-26
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Through the search, no patent publications related to

Method used

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  • Method for preparing ferritin-chitosan complex by utilizing transglutaminase and chitosan
  • Method for preparing ferritin-chitosan complex by utilizing transglutaminase and chitosan
  • Method for preparing ferritin-chitosan complex by utilizing transglutaminase and chitosan

Examples

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Embodiment 1

[0034] A method utilizing transglutaminase and chitosan to prepare ferritin-chitosan complex, the steps are as follows:

[0035] Using commercially available red beans as raw materials, ferritin is extracted and separated by water extraction, chitosan is covalently bound to ferritin by the catalysis of transglutaminase, and effective binding components are separated by gel filtration , the obtained ferritin-chitosan complex. The heat stability of the complex and the ability to resist gastrointestinal digestive enzymes are significantly enhanced.

[0036] Preferably, the specific steps are as follows:

[0037] (1) Use commercially available red beans as raw materials, soak in distilled water at 4°C overnight (about 10-12h), after peeling, add 2 times the volume of 50mM KH containing 1% PVP2 PO 4 -Na 2 HPO 4 (pH 7.0), and homogenized 3 times with an in-cut homogenizer, each time for 2-3 minutes, and filtered the bean dregs with a 200-mesh filter. Centrifuge the collected ho...

Embodiment 2

[0046] A method utilizing transglutaminase and chitosan to prepare ferritin-chitosan complex, the steps are as follows:

[0047] The method uses ferritin extracted from soybeans as a raw material, utilizes the catalysis of transglutaminase to bind chitosan to ferritin, and separates effective binding components through gel filtration to obtain ferritin-chitosan The obtained ferritin-chitosan complex has higher stability than ferritin.

[0048] Step by step, the specific steps are as follows:

[0049] ⑴Using red beans as raw materials, soak in distilled water at 4°C overnight (about 10-12h), after manual peeling, add 2 times the volume of 50mM KH containing 1% PVP 2 PO 4 -Na 2 HPO 4 (pH 7.0), and homogenized 3 times with an in-cut homogenizer, each time for 2-3 minutes, and filtered the bean dregs with a 200-mesh filter. Centrifuge the collected homogenate at 6000g at 4°C for 10-15 minutes, discard the precipitate, and obtain the crude protein supernatant;

[0050] (2) Ad...

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Abstract

The invention relates to a method for preparing a ferritin-chitosan complex by utilizing transglutaminase and chitosan. The method comprises the following steps: taking ferritin extracted from red beans as a raw material, binding chitosan onto the ferritin by utilizing a catalytic action of the transglutaminase, and separating effective binding components by a gel filtration method, thereby obtaining the ferritin-chitosan complex. The prepared ferritin-chitosan complex has high stability compared with the ferritin. According to the method, the chitosan is catalyzed to be covalently bound ontothe ferritin shell by utilizing the transglutaminase, the heat stability and gastrointestinal digestion stability of the ferritin are improved, and the bioavailability and iron supplement efficiency of the ferritin are further improved. The ferritin-chitosan complex prepared by the method can be applied to the industries such as food additives, feed, health foods and the like.

Description

technical field [0001] The invention belongs to the technical field of food, in particular to a method for preparing ferritin-chitosan complex by using transglutaminase and chitosan. Background technique [0002] Iron is an essential nutrient element for the human body. Nearly half of the world's population suffers from iron deficiency, which will affect people's health in severe cases. Iron deficiency anemia is one of the nutritional diseases with the highest incidence rate in the world today. However, due to the interference of small molecule chelating agents, the traditional iron supplementation preparations lead to unsatisfactory iron supplementation effects; at the same time, they will also have toxic and side effects on the human body and are gradually banned from use. Therefore, it is imminent to find a natural, non-toxic, biosoluble iron supplement product. [0003] Ferritin, the major form of endogenous iron in living organisms, can store large amounts of iron ato...

Claims

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Application Information

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IPC IPC(8): C12P21/02C07K14/79
CPCC12P21/02C07K14/79
Inventor 杨瑞刘玉茜王德生周中凯
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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