Application of ochratoxin A anti-idiotypic nano-antibody used as ochratoxin A standard substitute

An ochratoxin, anti-idiotype technology, applied in the field of molecular biology, can solve the problems of long production cycle, small antibody molecular weight, complex preparation process, etc., and achieve the effect of good accuracy and accurate results
CN109535256AActive Publication Date: 2019-03-29深圳市金阅检测科技有限责任公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
深圳市金阅检测科技有限责任公司
Publication Date
2019-03-29

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Abstract

The invention belongs to the field of molecular biology, and particularly relates to an application of an ochratoxin A anti-idiotypic nano-antibody used as a ochratoxin A standard substitute. The present invention obtains the ochratoxin A anti-idiotypic nano-antibody with an amino acid sequence shown in SEQ ID NO: 1. According to ELISA standard curves of standard ochratoxin A and an ochratoxin A anti-idiotypic antibody, under the same inhibition ratio, corresponding relationship curves of the concentration of the ochratoxin A and the concentration of the ochratoxin A anti-idiotypic antibody are established. Besides, the results are subjected to exponential regression analysis to obtain a corresponding relationship between the the ochratoxin A and the ochratoxin A anti-idiotypic antibody. The corresponding relationship can be applied to the detection and analysis of ochratoxin A in agricultural products. The immunodetection method that the ochratoxin A anti-idiotypic nano-antibody replaces the standard ochratoxin A has accurate and reliable results, and is an effective and feasible method for green immunoassay.
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Description

technical field

[0001] The invention belongs to the field of molecular biology, and specifically relates to the application of an ochratoxin A anti-idiotype nanobody as a substitute for an ochratoxin A standard product. Background technique

[0002] Ochratoxin A (ocbratoxinA, OTA) is a toxic fungal secondary metabolite, mainly produced by Aspergillus and Penicillium, which mainly pollutes food, vegetables, coffee, grapes, cocoa and other economic crops. The globalization of toxin-producing fungi and the continuous development of world trade in crop products have resulted in the widespread spread of ochratoxin A in the world, and has seriously endangered the health of animals and humans. It has strong nephrotoxicity and hepatotoxicity, and has teratogenicity, carcinogenicity, immunotoxicity and embryotoxicity. Many countries have set limit standards, so it is extremely important to establish a low-cost, fast and effective on-site detection method.

[0003] At present, the me...

Claims

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