Method for preparing rapid detection element of biological hydrogen sulfide

Abstract

The invention discloses a method for preparing a rapid detection element of biological hydrogen sulfide, belongs to the technical field of biological hydrogen sulfide detection, and solves the problems such as heavy instruments, complicated operation of detection, long detection period and poor sensibility of existing detection elements. The method for preparing the rapid detection element of biological hydrogen sulfide comprises the following steps: taking a detection base, dropping an aqueous solution of cuprous oxide nanomaterials in a detection tank of the detection base, then dropping a polyvinyl alcohol solution in the detection tank after natural drying at the room temperature to cover the cuprous oxide nanomaterials, and putting the entire detection base into a freeze dryer for freeze-drying, thereby obtaining the rapid detection element of biological hydrogen sulfide. The method provided by the invention can be used to prepare the rapid detection element of biological hydrogensulfide.

Description

technical field

[0001] The invention relates to a preparation method of a biological hydrogen sulfide rapid detection element, belonging to the technical field of biological hydrogen sulfide detection. Background technique

[0002] H in vivo 2 The content of S will change significantly with the pathological changes of various diseases such as respiratory tract and coronary heart disease, so the H in the organism can be 2 The content of S serves as a detection marker for these diseases. Realization of in vivo H 2 The rapid and convenient detection of S concentration can achieve the purpose of timely monitoring of diseases, which is very beneficial for patients (especially for patients with incurable chronic diseases) to grasp the condition in time, and is of great significance to human health.

[0003] At present, the main technologies for detecting hydrogen sulfide in vivo include: mass spectrometry, spectrophotometry, electrochemical method, fluorescent probe method, hig...

Examples

Embodiment 1

[0025] The preparation method of described biological hydrogen sulfide rapid detection element comprises the following steps:

[0026] Add 0.450g of copper acetate and 3.981g of triethanolamine to a solution of 64mL of absolute ethanol and 96mL of water, put it on a magnetic stirrer and stir for 12min until the solution turns dark blue, add 0.052g of sodium hydroxide and 2.235 gPVP, after stirring for 13 minutes, add 5.374ml of glucose solution with a concentration of 1mol / L, and continue to stir, and then react the stirred solution at a constant temperature of 90°C for 7 hours to obtain cuprous oxide nanomaterials. Aqueous solution of copper nanomaterials.

[0027] Take the detection substrate, drop 0.007g / mL cuprous oxide nanomaterial aqueous solution into the detection tank 1 of the detection base, let it dry naturally at room temperature, and then drop 0.04g / mL polyvinyl alcohol solution into the detection tank 1 to cover the oxidation. Cuprous nanomaterials, and then put...

Embodiment 2

[0030] The preparation method of described biological hydrogen sulfide rapid detection element comprises the following steps:

[0031] Add 0.4g of copper acetate and 3g of triethanolamine to a solution of 64mL of absolute ethanol and 96mL of water, put it on a magnetic stirrer and stir for 10min until the solution turns dark blue, add 0.051g of sodium hydroxide and 2.1g of PVP under stirring conditions After stirring for 10 minutes, 5.3ml of glucose solution with a concentration of 1mol / L was added, and the stirring was continued. Then, the stirred solution was reacted at a constant temperature of 87°C for 6.5 hours. After washing, an aqueous solution of cuprous oxide nanomaterials was prepared.

[0032] Take the detection substrate, drop 0.002g / mL cuprous oxide nanomaterial aqueous solution into the detection tank 1 of the detection base, let it dry naturally at room temperature, and then drop 0.02g / mL polyvinyl alcohol solution into the detection tank 1 to cover the oxidation...

Embodiment 3

[0035] The preparation method of described biological hydrogen sulfide rapid detection element comprises the following steps:

[0036] Add 0.5g of copper acetate and 4g of triethanolamine to a solution of 64mL of absolute ethanol and 96mL of water, put it on a magnetic stirrer and stir for 15min until the solution turns dark blue, add 0.055g of sodium hydroxide and 2.3g of PVP under stirring conditions , after stirring for 15 minutes, add 5.5ml of glucose solution with a concentration of 1mol / L, and continue to stir, then react the stirred solution at a constant temperature of 92°C for 7.5 hours, wash it and prepare an aqueous solution of cuprous oxide nanomaterials.

[0037] Take the detection substrate, drop 0.01g / mL cuprous oxide nanomaterial aqueous solution into the detection tank 1 of the detection base, let it dry naturally at room temperature, and then drop 0.06g / mL polyvinyl alcohol solution into the detection tank 1 to cover the oxidation. Cuprous nanomaterials, and ...

technical field

[0001] The invention relates to a preparation method of a biological hydrogen sulfide rapid detection element, belonging to the technical field of biological hydrogen sulfide detection. Background technique

[0002] H in vivo 2 The content of S will change significantly with the pathological changes of various diseases such as respiratory tract and coronary heart disease, so the H in the organism can be 2 The content of S serves as a detection marker for these diseases. Realization of in vivo H 2 The rapid and convenient detection of S concentration can achieve the purpose of timely monitoring of diseases, which is very beneficial for patients (especially for patients with incurable chronic diseases) to grasp the condition in time, and is of great significance to human health.

[0003] At present, the main technologies for detecting hydrogen sulfide in vivo include: mass spectrometry, spectrophotometry, electrochemical method, fluorescent probe method, hig...

Examples

Embodiment 1

[0025] The preparation method of described biological hydrogen sulfide rapid detection element comprises the following steps:

[0026] Add 0.450g of copper acetate and 3.981g of triethanolamine to a solution of 64mL of absolute ethanol and 96mL of water, put it on a magnetic stirrer and stir for 12min until the solution turns dark blue, add 0.052g of sodium hydroxide and 2.235 gPVP, after stirring for 13 minutes, add 5.374ml of glucose solution with a concentration of 1mol / L, and continue to stir, and then react the stirred solution at a constant temperature of 90°C for 7 hours to obtain cuprous oxide nanomaterials. Aqueous solution of copper nanomaterials.

[0027] Take the detection substrate, drop 0.007g / mL cuprous oxide nanomaterial aqueous solution into the detection tank 1 of the detection base, let it dry naturally at room temperature, and then drop 0.04g / mL polyvinyl alcohol solution into the detection tank 1 to cover the oxidation. Cuprous nanomaterials, and then put...

Embodiment 2

[0030] The preparation method of described biological hydrogen sulfide rapid detection element comprises the following steps:

[0031] Add 0.4g of copper acetate and 3g of triethanolamine to a solution of 64mL of absolute ethanol and 96mL of water, put it on a magnetic stirrer and stir for 10min until the solution turns dark blue, add 0.051g of sodium hydroxide and 2.1g of PVP under stirring conditions After stirring for 10 minutes, 5.3ml of glucose solution with a concentration of 1mol / L was added, and the stirring was continued. Then, the stirred solution was reacted at a constant temperature of 87°C for 6.5 hours. After washing, an aqueous solution of cuprous oxide nanomaterials was prepared.

[0032] Take the detection substrate, drop 0.002g / mL cuprous oxide nanomaterial aqueous solution into the detection tank 1 of the detection base, let it dry naturally at room temperature, and then drop 0.02g / mL polyvinyl alcohol solution into the detection tank 1 to cover the oxidation...

Embodiment 3

[0035] The preparation method of described biological hydrogen sulfide rapid detection element comprises the following steps:

[0036] Add 0.5g of copper acetate and 4g of triethanolamine to a solution of 64mL of absolute ethanol and 96mL of water, put it on a magnetic stirrer and stir for 15min until the solution turns dark blue, add 0.055g of sodium hydroxide and 2.3g of PVP under stirring conditions , after stirring for 15 minutes, add 5.5ml of glucose solution with a concentration of 1mol / L, and continue to stir, then react the stirred solution at a constant temperature of 92°C for 7.5 hours, wash it and prepare an aqueous solution of cuprous oxide nanomaterials.

[0037] Take the detection substrate, drop 0.01g / mL cuprous oxide nanomaterial aqueous solution into the detection tank 1 of the detection base, let it dry naturally at room temperature, and then drop 0.06g / mL polyvinyl alcohol solution into the detection tank 1 to cover the oxidation. Cuprous nanomaterials, and ...

Images