Magnetorheological fluid technology based isothermal amplification type tera-hertz multichannel microfluidic chip and method thereof for detecting pathogenic bacteria

Abstract

The invention discloses a magnetorheological fluid technology based isothermal amplification type tera-hertz multichannel microfluidic chip and a method thereof for detecting pathogenic bacteria. Thechip comprises a rolling circle amplification (RCA) reaction module, a magnetorheological fluid switching module, a metamaterial nanoparticle signal amplification module and a microfluid chip structure. The microfluid chip structure is of a compass-type structure with a plurality of detection channels, a total inlet is formed in the center of the microfluid chip structure, and each detection channel is sequentially connected with the RCA reaction module, the magnetorheological fluid switching module and the metamaterial nanoparticle signal amplification module from inside to outside. Accordingto the detection method, the problem that water susceptibility and sensitivity are low is solved by using RCA, magnetorheological fluid and the metamaterial nanoparticle signal amplification module.As a novel method for rapidly and accurately detecting the pathogenic bacteria, the detection method has an important significance in diagnosis and treatment of infectious diseases.

Description

technical field [0001] The invention relates to the field of pathogenic bacteria detection, in particular to a constant temperature amplification terahertz multi-channel microfluidic chip based on magnetorheological fluid technology, and also to a method for detecting pathogenic bacteria by using the microfluidic chip. Background technique [0002] Rapid and accurate detection of the species of pathogenic bacteria is crucial for the diagnosis and subsequent treatment of infectious diseases. Traditional pathogenic bacteria detection methods take a long time, from collecting specimens to accurately obtaining the type of pathogenic bacteria usually takes a few days to a week or so. In addition, the bacterial culture operation is cumbersome, and different bacteria require different enzymatic and biochemical reaction identifications, which consume a large amount of reagents. In order to quickly identify the species of pathogenic bacteria, a series of molecular biology methods in...

AI Technical Summary

Problems solved by technology

However, there are still problems of specificity, sensitivity and water sensitivity in the detection of pathogenic bacteria by using the existing THz wave detection technology.

(1) Specificity problem: The direct detection of pathogenic bacteria by THz waves is based on the principle that different species of pathogenic bacteria have different intracellular water content and intracellular structure. Although this method can distinguish pathogenic bacteria after purification and culture, it is essentially not Does not have high detection specificity

Considering that there are pathogenic bacteria with similar results or mixed with multiple pathogenic bacteria in clinical samples, the direct detection method cannot specifically distinguish the species of pathogenic bacteria in clinical samples

(2) Sensitivity problem: the previous research results show that without any signal amplification technology, THz spectrum can only detect 10 8 Pathogens above CFU

(3) Water sensitivity problem: at room temperature, the absorption coefficient of water to THz waves at 1THz frequency is as high as 250cm -1 left and right, the THz absorption signal of pathogenic bacteria in the liquid phase environment is often annihilated

But so far, there is no report on the application of magnetorheological fluid technology in the field of terahertz wave detection.

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