The application of cyp306a1 gene, its encoded protein and its dsrna in pest control of Gypsy gypsy moth
A technology of gene coding and gypsy moth, applied in the field of molecular biology, can solve the problem of gypsy moth's drug resistance and pollution of the environment
Active Publication Date: 2022-03-18
HEZE UNIV
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AI Technical Summary
Problems solved by technology
[0004] The present invention aims to solve the problems of drug resistance and environmental pollution in the existing chemical control of gypsy moth, and provides an application of the gypsy moth CYP306a1 gene, its encoded protein and its dsRNA in pest control
Method used
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specific Embodiment approach 1
[0034] Embodiment 1: The nucleotide sequence of the CYP306a1 gene of the gypsy moth in this embodiment is shown in SEQ ID NO: 1 in the sequence listing. The nucleotide sequence of its coding region CDS is shown in SEQ ID NO: 2 in the sequence listing.
specific Embodiment approach 2
[0035] Specific embodiment 2: The amino acid sequence of the protein encoded by the CDS of the coding region of the CYP306a1 gene of the gypsy moth CYP306a1 in this embodiment is shown in SEQ ID NO: 3 in the sequence listing.
specific Embodiment approach 3
[0036] Specific embodiment three: The dsRNA sequence of the CYP306a1 gene of the gypsy moth in this embodiment is shown in SEQ ID NO: 4 in the sequence listing.
[0037] In this embodiment, the CYP306a1 gene fragment of the gypsy moth is used as a template, and a specific dsRNA primer pair is designed to synthesize the CYP306a1 gene dsRNA through the MEGAscript RNAi kit (Ambion).
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Abstract
The application of gypsy moth CYP306a1 gene, its encoded protein and its dsRNA in pest control relates to the field of molecular biology, in particular to the gypsy moth CYP306a1 gene, its encoded protein and its application. The invention aims to solve the problems of drug resistance and environmental pollution in the existing chemical control of the gypsy moth. The nucleotide sequence of the gene is shown as SEQ ID NO: 1 in the sequence listing. The amino acid sequence of the protein encoded by the CDS of its coding region is shown in SEQ ID NO: 3 in the sequence listing. The invention interferes with the gene CYP306a1 of the gypsy moth and silences the gene, thereby prolonging the molting period of the larvae, slowing down the development, and even causing death. The invention is used in the field of gypsy moth pest control.
Description
technical field [0001] The invention relates to the field of molecular biology, in particular to the gypsy moth CYP306a1 gene, its encoded protein and its application. Background technique [0002] The gypsy moth (Lymantria dispar Linnaeus) is an important agricultural and forestry foliage pest, which has the characteristics of a wide distribution range, a variety of feeding plants, fast transmission, large reproduction and periodic outbreaks. According to domestic reports, the gypsy moth can harm more than 500 kinds of plants, which not only causes huge economic losses to forestry, but also damages its ecological benefits. At present, although natural enemy microorganisms, insects, or physical methods are used in the control of gypsy moth pests, the control effect on this explosive pest is not significant. Chemical pesticides are used in the control of gypsy moth pests due to their high-efficiency and rapid insecticide characteristics. Prevention and control is still the m...
Claims
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IPC IPC(8): C12N15/12C12N15/113C07K14/435A01N57/16A01P7/04
CPCA01N57/16C12N15/113C07K14/43563
Inventor 王步勇问荣荣刘玉梅孙西寨
Owner HEZE UNIV