Dedicated diluent for detecting streptococcus pneumoniae in urine sample

Abstract

The invention discloses a dedicated diluent for detecting streptococcus pneumoniae in a urine sample. The diluent consists of five ingredients, including borax, 1307Prill, [Epsilon]-polylysine, a C-reactive protein polyclonal antibody and purified water, wherein the content of each ingredient is as follows: the content of the borax which serves as a first ingredient is 50-100mM, the content of 1307Prill which serves as a second ingredient is 0.5-1%, the content of the [Epsilon]-polylysine which serves as a third ingredient is 0.5-1%, the content of the C-reactive protein polyclonal antibody which serves as a fourth ingredient is 10-20ug / ml, and the balance is purified water. After the diluent is mixed with the urine sample at a volume ratio of 1:9, the interference of C-reactive protein inthe urine sample for a streptococcus pneumoniae detection result can be effectively inhibited, the detection rate of the streptococcus pneumoniae is obviously improved so as to perform significance for accurately detecting the streptococcus pneumoniae in the urine sample, and therefore, the diluent has a good clinical application value.

Description

technical field [0001] The invention relates to a special diluent for detecting Streptococcus pneumoniae in urine samples, which can quickly and effectively dissociate the complex of C-reactive protein (CRP) in urine samples and C-polysaccharide of Streptococcus pneumoniae cell wall, and at the same time inhibit the formation of CRP Biological activity belongs to the application field of immunology, and is suitable for immunological experimental methods such as immunochromatography, immunodiafiltration, enzyme-linked immunosorbent assay, immunoturbidimetry, and chemiluminescence. Background technique [0002] Streptococcus pneumoniae is the main pathogen causing bacterial pneumonia. Pneumococcal pneumonia has a case-fatality rate of up to 30%, depending on bacteremia, age, and underlying disease. If not properly diagnosed and treated, pneumococcal infection can lead to bacteremia, meningitis, pericarditis, empyema, purpura fulminans, endocarditis, and / or arthritis. People ...

AI Technical Summary

Problems solved by technology

[0006] Through density gradient centrifugation, gel electrophoresis, western blotting and other technical means, we found that CRP was combined with the C-polysaccharide antigen of Streptococcus pneumoniae in the urine of patients infected with Streptococcus pneumoniae, so we speculated that this combination may be effective for Streptococcus pneumoniae. The detection of S. pneumoniae caused interference, which in turn affected the detection rate of Streptococcus pneumoniae