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A kind of arid5b gene mutation site and its application affecting the intramuscular fat content of beef cattle

An ARID5B-HRM-F10, intramuscular fat technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems that have not yet been reported in research

Active Publication Date: 2022-03-15
JILIN ACAD OF AGRI SCI
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Problems solved by technology

It has been confirmed that the ARID5B gene is involved in fatty acid metabolism, but there is no report on the gene's effect on beef quality

Method used

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  • A kind of arid5b gene mutation site and its application affecting the intramuscular fat content of beef cattle
  • A kind of arid5b gene mutation site and its application affecting the intramuscular fat content of beef cattle
  • A kind of arid5b gene mutation site and its application affecting the intramuscular fat content of beef cattle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] 1 Materials and methods

[0024] 1.1 Test animals

[0025] 99 18-month-old Yanhuang cows were selected, and the intramuscular fat content was measured according to the standard of the beef cattle genetic improvement program (obtained by Gandalf veterinary B-ultrasound measurement). About 1.5 g of ear tissue was collected and put into a 2 mL EP tube containing 75% ethanol, marked and dated, brought back to the laboratory, and stored at -20°C.

[0026] 1.2 Main reagents and instruments

[0027] 480HRM Master Mix kit was purchased from Roche, USA; 2×ES TaqMasterMix was purchased from CWBIO; AxyPrep Genomic DNA Extraction Kit was purchased from Axygeno; PCR instrument T100 was purchased from Shanghai Bole Life Medical Products Co., Ltd.; -Q5000 was purchased from Beijing Dingsheng Biotechnology Co., Ltd.

[0028] 1.3 Genomic DNA extraction

[0029] Genomic DNA was extracted from ear tissues of 99 Yanhuang Cattle according to the instructions of the AxyPrep Genomic DNA...

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Abstract

The invention discloses an ARID5B gene mutation site which affects the intramuscular fat content of beef cattle, which is located at the 261 bp of exon 10 of the ARID5B gene. The invention uses the Yanhuang cattle DNA as a template, detects the polymorphism of the ARID5B gene through the HRM typing technique, and then analyzes the correlation between the polymorphism of the gene and the traits of the Yanhuang beef through single factor analysis of variance. The results of the invention can provide a reference for the quality improvement of Yanhuang beef and the screening of effective genetic marker genes.

Description

technical field [0001] The invention relates to the technical field of Yanhuang cattle breed breeding, in particular to an ARID5B gene mutation site that affects the intramuscular fat content of beef cattle and its application. Background technique [0002] Yanhuang cattle have attracted more and more attention because of their advantages such as strong adaptability, fast growth, high meat yield and unique meat flavor. It is one of the leading beef cattle breeds raised in Yanji City, Jilin Province. The source of the breed of Yanhuang cattle is a new type of cultivar produced by crossing Yanhuang cattle and Limousin after 27 years of hybridization, cross-crossing fixation and group selection. [0003] The AT-rich interactive domain 5B (ARID5B) gene is located on bovine chromosome 28, contains 13 exons, and encodes a total of 939 amino acids. It is an important member of the ARID gene family. ARID contains 15 human-specific proteins, which can be divided into seven subtypes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11C12N15/12
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 曹阳赵玉民吴健胡忠昌金海国于永生张立春肖成魏天高一
Owner JILIN ACAD OF AGRI SCI
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