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Selenium enriched saccharomyces cerevisiae, saccharomyces cerevisiae rich in selenium and lycopene, and preparation method thereof

A selenium-enriched Saccharomyces cerevisiae, Saccharomyces cerevisiae technology, applied in biochemical equipment and methods, microbial-based methods, fermentation, etc., can solve the problems of single nutrition, little increase in organic selenium, insufficient resource utilization, etc., and achieve selenium conversion The effect of ability improvement

Active Publication Date: 2019-04-30
CABIO BIOTECH WUHAN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the organic selenium in yeast exists in the form of selenomethionine, but in the prior art, it is rare to increase the content of organic selenium by increasing the expression content of methionine in yeast
[0005] CN106566779A constructed a recombinant Saccharomyces cerevisiae that produces lycopene, but the recombinant yeast strain only produces lycopene, the product is single, the nutrition is single, the resource utilization is insufficient, and the obtained lycopene products lack selenium or their content is low

Method used

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  • Selenium enriched saccharomyces cerevisiae, saccharomyces cerevisiae rich in selenium and lycopene, and preparation method thereof
  • Selenium enriched saccharomyces cerevisiae, saccharomyces cerevisiae rich in selenium and lycopene, and preparation method thereof
  • Selenium enriched saccharomyces cerevisiae, saccharomyces cerevisiae rich in selenium and lycopene, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The method for preparing selenium-enriched lycopene products provided in this embodiment is as follows:

[0050] The strain used: Saccharomyces cerevisiae, the preservation number is CGMCC NO.13013.

[0051] 1 strain activation:

[0052] After the strains were streaked on the plate in the slant medium, they were activated and cultured in a constant temperature incubator at 30°C for 24-36h.

[0053] Among them, the slant medium contains (g / L): 20 glucose, 10 yeast extract, 20 peptone, 20 agar, and sterilized at 121° C. for 20 minutes.

[0054] 2 seed cultures:

[0055] The strains after the activation culture were transferred to the seed culture medium, the culture temperature was 30° C., the rotation speed was 250 rpm, and the cultured for 12-16 hours was used as the seed liquid.

[0056] Seed medium (g / L): 20 glucose, 10 yeast extract, 20 peptone, 50 mg / L uracil, sterilized at 121°C for 20 minutes.

[0057] 3 fermentation:

[0058] The activated seed solution was in...

Embodiment 2-6

[0094] The method for preparing selenium-enriched lycopene products provided in Examples 2-6 is basically the same as that in Example 1, and the different parameters are shown in Table 1 below.

[0095] Table 1

[0096]

[0097]

[0098] Wherein, "-" represents that ultrasonic treatment was not performed in Example 6.

Embodiment 7

[0100] In this example, common Saccharomyces cerevisiae was purchased from Guangdong Huankai Company. The Saccharomyces cerevisiae standard strain ATC9763, and the process and parameters of selenium enrichment were the same as in Example 1.

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Abstract

The invention discloses selenium enriched saccharomyces cerevisiae, saccharomyces cerevisiae rich in selenium and lycopene, and a preparation method thereof, and relates to the technology of lycopenefermentation. According to the disclosed selenium enriched saccharomyces cerevisiae preparation method, a surfactant is added into a fermentation culture medium with inoculated saccharomyces cerevisiae, and the preparation method can increase the selenium content of saccharomyces cerevisiae.

Description

technical field [0001] The invention relates to the technical field of lycopene fermentation, in particular to a selenium-enriched Saccharomyces cerevisiae, a selenium-enriched lycopene-enriched Saccharomyces cerevisiae and a preparation method thereof. Background technique [0002] Lycopene is a natural pigment found in plants. It is mainly found in the ripe fruit of tomato, a plant of the family Solanaceae. It is one of the strongest antioxidants currently found in plants in nature. It has been scientifically proven that singlet oxygen and oxygen free radicals in the human body are the chief culprits of invading the human body's own immune system. Lycopene is far superior to other carotenoids and vitamin E in scavenging free radicals, and its singlet oxygen quenching rate constant is 100 times that of vitamin E. It can effectively prevent and treat various diseases caused by aging and decreased immunity. Therefore, it has attracted the attention of experts from all ove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12N1/38C12P5/02C12R1/865
CPCC12N1/18C12N1/38C12P5/007Y02E50/10
Inventor 元英进汪志明李翔宇陆姝欢杨艳红余超
Owner CABIO BIOTECH WUHAN CO LTD
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