Selenium enriched saccharomyces cerevisiae, saccharomyces cerevisiae rich in selenium and lycopene, and preparation method thereof
A selenium-enriched Saccharomyces cerevisiae, Saccharomyces cerevisiae technology, applied in biochemical equipment and methods, microbial-based methods, fermentation, etc., can solve the problems of single nutrition, little increase in organic selenium, insufficient resource utilization, etc., and achieve selenium conversion The effect of ability improvement
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Embodiment 1
[0049] The method for preparing selenium-enriched lycopene products provided in this embodiment is as follows:
[0050] The strain used: Saccharomyces cerevisiae, the preservation number is CGMCC NO.13013.
[0051] 1 strain activation:
[0052] After the strains were streaked on the plate in the slant medium, they were activated and cultured in a constant temperature incubator at 30°C for 24-36h.
[0053] Among them, the slant medium contains (g / L): 20 glucose, 10 yeast extract, 20 peptone, 20 agar, and sterilized at 121° C. for 20 minutes.
[0054] 2 seed cultures:
[0055] The strains after the activation culture were transferred to the seed culture medium, the culture temperature was 30° C., the rotation speed was 250 rpm, and the cultured for 12-16 hours was used as the seed liquid.
[0056] Seed medium (g / L): 20 glucose, 10 yeast extract, 20 peptone, 50 mg / L uracil, sterilized at 121°C for 20 minutes.
[0057] 3 fermentation:
[0058] The activated seed solution was in...
Embodiment 2-6
[0094] The method for preparing selenium-enriched lycopene products provided in Examples 2-6 is basically the same as that in Example 1, and the different parameters are shown in Table 1 below.
[0095] Table 1
[0096]
[0097]
[0098] Wherein, "-" represents that ultrasonic treatment was not performed in Example 6.
Embodiment 7
[0100] In this example, common Saccharomyces cerevisiae was purchased from Guangdong Huankai Company. The Saccharomyces cerevisiae standard strain ATC9763, and the process and parameters of selenium enrichment were the same as in Example 1.
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