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Detection Method of Fingerprint of Hawthorn Particles
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A fingerprint and detection method technology, applied in the field of detection of Chinese patent medicines, to achieve high precision, stable baseline, and good stability
Active Publication Date: 2020-12-25
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1
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At present, there are few detection methods about the fingerprints of the big hawthorn granules. The present invention adopts high performance liquid chromatography to establish the fingerprint detection method of the big hawthorn granules, and accurately analyzes the active ingredients in it, so as to comprehensively and objectively evaluate the quality of the big hawthorn granules. of great significance
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Embodiment 1
[0043] Embodiment 1 A kind of detection method of big hawthorn grain fingerprint, comprises the following steps:
[0044] Step (1), the preparation of Dashanzha granule test solution:
[0045] Take the 10 batches of hawthorn granules in the above table 1, grind them thoroughly, weigh 20g, place them in a 100mL conical flask, accurately absorb 40mL of methanol solution, ultrasonicate for 30min, take the supernatant after filtration, add methanol solution to make up the weight , to get 10 batches of test solution;
[0046] Step (2), the preparation of reference substance solution
[0047] Get a certain amount of ursolic acid reference substance, place it in the volumetric flask after accurately weighing, add methanol solution to dissolve and be settled to the mark, and the obtained concentration is 0.5mg / mL ursolic acid reference substance solution;
[0048]Step (3), each batch of need testing solution and reference substance solution of step 1 is precisely drawn respectively,...
Embodiment 2
[0060] Embodiment 2: methodological investigation
[0061] 1. Precision experiment
[0062] Accurately draw the need testing solution of the 170806 batch number in embodiment 1, continuously sample injection 6 times by the chromatographic conditions under embodiment 1, the RSD of its main total peak retention time is all less than 0.41%, and the relative peak area RSD is all less than 2.86%, It shows that the precision of the instrument is good. The specific experimental results are shown in Table 6 and Table 7.
[0066] serial number peak 1 peak 2 peak 3 1 8593095 3816466 3...
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Abstract
The invention discloses a method for detecting fingerprint of hawthorn granules. The method comprises the following steps: 1, preparing a sample solution; 2, preparing a reference solution; 3, precisely absorbing the sample solution, injecting into a liquid chromatograph, and recording a chromatogram; and 4, deriving a hawthorn granule fingerprint obtained in the step 3 by an instrument, introducing into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, and selecting chromatographic peaks in chromatograms of different batches of hawthorn granules as common peaks; calculating the comparative fingerprint of the hawthorn fingerprint by means of an average calculation method; and calculating the relative retention time and relative peak area of the commonpeaks. The hawthorn fingerprint provided by the invention can comprehensively and objectively characterize the quality of the hawthorn granules. The detection method of the fingerprint provided by the invention has the advantages of easiness, convenience, stability, high precision and high reproducibility.
Description
technical field [0001] The invention relates to a detection method of a Chinese patent medicine, in particular to a detection method of the fingerprint of hawthorn granules. Background technique [0002] Fingerprint refers to certain complex substances, such as traditional Chinese medicine, or the DNA of certain organisms or certain tissues or cells. After proper processing of proteins, certain analytical methods are used to obtain chromatograms or spectra that can indicate their chemical characteristics. picture. TCM fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of TCM and is mainly used to evaluate the authenticity, goodness and stability of the quality of TCM materials and TCM preparations. Traditional Chinese medicine and its preparations are multi-component complex systems, so the evaluation of its quality should adopt a detection method that is suitable for it and can provide ...
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