Fluorescent standard card and manufacturing method of fluorescent film used for fluorescent standard card

Abstract

The invention discloses a fluorescent standard card. The fluorescent standard card comprises a card shell and a container, a shading board sleeves the card shell, and the container is arranged on oneside of the shading board; the bottom outer wall of the container is attached to one side of the shading board; a plurality of groups of windows are arranged on the shading board; and a fluorescent film is arranged on the bottom inner wall of the container. According to the fluorescence standard card, the fluorescence property is stable, the structure is simple and practical, the fluorescence standard card can realize the calibration of a fluorescent immunoassay instrument, and can be stable for a long time.

Description

technical field [0001] The invention belongs to the field of instrument calibration, in particular to a fluorescent standard card and a method for preparing a fluorescent film for the fluorescent standard card. Background technique [0002] At present, fluorescent immunoassay instruments based on fluorescent materials usually use a light source with a certain wavelength and intensity to irradiate the markers on the fluorescent film to fluoresce when performing project detection, and then convert the fluorescent signal into an electrical signal and draw a fitting curve , Calculate the area or intensity of the peak of the curve to determine the intensity of the corresponding fluorescent signal, thereby establishing the relationship between the electrical signal and the concentration of the measured sample. [0003] However, in the process of determining the fluorescence intensity of the fluorescent immunoassay instrument based on fluorescence, due to the sensor inside the fluo...

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Problems solved by technology

[0003] However, in the process of determining the fluorescence intensity of the fluorescent immunoassay instrument based on fluorescence, due to the sensor inside the fluorescent immunoassay instrument, it is affected by factors such as the strength of the signal source and the use environment during the working period, and the instrument is used for a long time. , due to equipment damage and aging, the test results have a large deviation

Therefore, before a project is tested, if there is a lack of calibration of the fluorescent immunoassay instrument, it will affect the consistency and accuracy of the instrument's measurement results

At present, there is no fluorescent standard card specially used for the calibration of fluorescent immunoassay instruments and various bioanalytical detectors based on fluorescent materials such as in situ hybridization and biochips.

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