A method and product for preparing autofluorescent polystyrene material

Abstract

The invention provides a method and product for preparing autofluorescence polystyrene material, which is characterized in that on the surface of polystyrene material, especially on the surface of polystyrene microspheres, it is acylated by Friedel-Crafts under the action of an acidic catalyst (Alkylation) reaction introduces small molecule color-producing (color-aiding) functional groups, which form π-π (p-π) conjugation with the large π bond of the benzene ring to form a conjugated system, which undergoes electronic transition under the action of excitation light Produce fluorescence. Under the excitation of 405nm laser, the prepared polystyrene fluorescent microspheres can produce obvious fluorescent signals in the blue, green and orange channels of the laser confocal microscope. At the same time, we found that the introduction of electron-withdrawing groups on the conjugated substituents can red-shift the emission peak of polystyrene materials. The preparation method of the invention is simple, high in efficiency and low in cost. The prepared autofluorescent polystyrene microspheres have the characteristics of high fluorescence intensity, stable chemical properties, no fluorescence leakage, high mechanical strength, etc., and have great application potential in the fields of biomedicine and electronic light-emitting devices.

Description

technical field [0001] The invention relates to a method and product for preparing autofluorescent polystyrene materials, especially to functionalize the surface of polystyrene microspheres, and the obtained microspheres can emit fluorescence under the excitation of ultraviolet light, and are used in biomedicine and light-emitting devices. and other fields have great application potential, and belong to the field of preparation of functional polymer materials. Background technique [0002] Organic fluorescent polymer materials are research hotspots in the field of materials science in recent years (Anal. Lett., 2000, 33, 3183-3197; Macromol. Chem. Phys., 2002, 203, 1833-1843), and have been used in various technical fields Especially in the field of biomedical detection, it has been widely used. Compared with fluorescent small molecules, fluorescent polymer materials have the advantages of uniform distribution of chromophores, stable content, and good luminescence performan...

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Problems solved by technology

The preparation method of the adsorption method is simple, but the dyes on the surface of the fluorescent microspheres are easy to fall off; the embedding method is to disperse the dyes evenly in the monomer, and prepare them by polymerization reaction or layer-by-layer self-assembly. The obtained microspheres generally have a core-shell structure. The fluorescent properties of the obtained microspheres are stable, but there is a problem of dye leakage; the chemical coupling method is to bond the dye molecules to the surface of the microspheres through chemical reactions, which is easily limited by the number of binding sites on the surface of the microspheres, and the fluorescent dye molecules are easily affected by Environmental interference; the copolymerization method refers to fluorescent microspheres prepared by polymerizing fluorescent substances with polymerizable functional groups and organic monomers, and the fluorescent groups are evenly distributed and difficult to leak (Adv.Mater.,2012,24:637-641) , but the problem of the copolymerization method is that it is easy to produce chain transfer reaction and it is difficult to obtain high molecular weight polymers.

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