Micro-droplet detecting chip
A technology for detecting chips and micro-droplets, applied in laboratory appliances, laboratory containers, chemical instruments and methods, etc., can solve the problem of high cost of batch processing of micro-droplet chips, unsuitability for clinical testing applications, and cumbersome manual operations Process and other issues, to achieve the effect of low cost of batch processing
Pending Publication Date: 2019-05-14
TARGETINGONE CORP +1
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Problems solved by technology
Hand-processed PDMS droplet chip has poor reliability
Batch processing of PDMS droplet chips is expensive
[0006] (2) PDMS micro-droplet chip sample injection and droplet collection are cumbersome manual procedures, which are not suitable for clinical testing applications
Method used
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[0035] In order to enable those skilled in the art to better understand the technical solutions in the application, the present invention will be further described below in conjunction with the embodiments. Obviously, the described embodiments are only a part of the embodiments of the application, rather than all embodiments . Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts shall fall within the scope of protection of this application. The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0036] Such as figure 1 Shown is a schematic diagram of the micro-droplet detection chip structure. Such as figure 1 As shown, in a standard optical disc with an outer diameter of 118 mm and an inner diameter of 22 mm, eight identical micro-droplet detection units 1 are arranged at equal intervals in a standard optical disc fr...
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The invention provides a micro-droplet detecting chip. The micro-droplet detecting chip comprises a central hole and is characterized in that the central hole is used for injecting an injection molding material during micro-droplet detecting chip production and the operation of the micro-droplet detecting chip during batch production and / or batch fluorescence detection; one or more micro-droplet detecting units are arranged on two sides of the central hole by using the central hole as the center. The micro-droplet detecting chip combining with the mature optical disc production process in theindustrial community has the advantages that the micro-droplet detecting chip can fast and reliably detect uniform micrometer-scale 'water in oil' micro-droplets, the micro-droplet detecting chip adopting a thermoplastic material is low in material and batch processing cost, a traditional annular optical disc structure is corrected, and optical disc space is utilized to the maximum extent.
Description
technical field [0001] The invention relates to the technical field of micro-droplet digital PCR, in particular to a micro-droplet detection chip. Background technique [0002] Droplet digital PCR (ddPCR) is a technique for absolute quantitative analysis of nucleic acids based on single-molecule PCR. Micro-droplet digital PCR technology is becoming the next revolutionary technology in the industry due to its advantages of high sensitivity and high accuracy. In recent years, with the development of micro-nano fabrication technology and microfluidics technology (micro-nanofabrication and microfluidics), micro-droplet digital PCR technology has encountered the best opportunity to break through the technical bottleneck. This technology uses a microfluidic chip to detect droplets with a diameter of several microns to hundreds of microns; the microdroplets wrap single molecules or single cells to achieve fully closed and fully integrated reaction and detection. The working princ...
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Inventor 荆高山王博苏世圣
Owner TARGETINGONE CORP