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Method of quickly decomposing green straws of plant and preventing and controlling root knot nematode disease of flue-cured tobacco

A root-knot nematode disease and straw technology, applied in botany equipment and methods, microbial-based methods, biochemical equipment and methods, etc. To achieve the effect of improving the soil texture, accelerating the rate of decomposition, and increasing the temperature

Inactive Publication Date: 2019-05-14
HONGHEZHOU BRANCH OF YUNNAN TOBACCO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purpose of the present invention is to select root-knot nematode disease prevention and control effect in the field of green straw to solve the problems of slow decomposing speed of green straw in the field, poor decomposing degree, easy to burn seedlings at the initial stage of transplanting, inability to effectively provide nutrients to seedlings, and poor control effect of root-knot nematode disease during crop cultivation. The oily radish with nematode disease is used as the green straw of green manure, and four kinds of microorganisms that have the effect of preventing and controlling root-knot nematode and decompose the green straw quickly and efficiently. The decomposing bacteria agent is sprayed on the green radish stalks in the blooming stage with the appropriate concentration and ratio of the four kinds of bacteria agents, and the straw after spraying the bacteria agent is further buried and decomposed on the spot for more than 60 days, which not only promotes the green straw Rapid and efficient decomposition, and effective synergistic control of root-knot nematodes in crop cultivation, and provide organic nutrients for crops

Method used

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  • Method of quickly decomposing green straws of plant and preventing and controlling root knot nematode disease of flue-cured tobacco
  • Method of quickly decomposing green straws of plant and preventing and controlling root knot nematode disease of flue-cured tobacco
  • Method of quickly decomposing green straws of plant and preventing and controlling root knot nematode disease of flue-cured tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1: The preparation of two strains of bacteria decomposing microbes.

[0054] (1) Preparation of two strains of bacterial culture medium and preparation of primary and secondary seeds

[0055] 1) Preparation and inoculation of solid LB medium (first-class seeds)

[0056] A. According to the quantity of culture, weigh as follows: Yeast extract 0.5%, tryptone 1%, NaCl 1%, add 60% tap water, dissolve and mix well (if preparing 1000mL solid medium, add respectively: Yeast extract 5g, tryptone 10g, NaCl 10g, tap water 600mL), by adding 2M NaOH, adjust the pH value to about 7.2, then add tap water to make up the volume of the medium to be prepared (such as 1000mL).

[0057] B. Take a conical flask with a volume of 500mL, subpackage the culture solution by 200mL / bottle, weigh 1.8% agar powder (3.6g) and subpackage it in a 500mL conical flask, add 200mL / bottle of culture solution, and plug it with cotton Plug, and sterilize at 121°C for 20 minutes together with othe...

Embodiment 2

[0070] Embodiment 2: Preparation of two fungal decomposing agents.

[0071] (1) Preparation of two fungal culture media and preparation of primary and secondary seeds

[0072] Preparation of solid PDA medium and inoculation culture (first-level seeds) and preparation of second-level seeds.

[0073] A. According to the quantity of cultivation, prepare according to the following medium formula ratio (mass percentage): 20% of peeled fresh potatoes, cut it into small pieces, add 5 times of distilled water of potato chips (as preparing the solid PDA medium of 1000mL, then Use respectively: fresh potato 200g, distilled water 1000mL), heat and boil for 30min, wait until the temperature drops to room temperature, filter with double-layer gauze, then wash the boiled potato slices (mud) with distilled water and filter with double-layer gauze, collect the filtrate, mix Mix well, add 2% sucrose (20g), add water to make up to 1000mL, dissolve and mix, the pH value of the culture solution ...

Embodiment 3

[0089] Example 3: Seed sowing and green straw planting and production of oily radish in Jianshui County and Mengzi City.

[0090] (1) Radish seeds and implementation site

[0091] A. Oily radish seeds: Oily radish seeds are developed and preserved by the Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, and are jointly propagated with Yunnan Tobacco Company Honghezhou Company.

[0092] B. Implementation location and area: Mingshao Village, Lin'an Town, Jianshui County and Dupan Village, Mingjiu Town, Mengzi City, each with an area of ​​5 mu.

[0093] (2) Sowing and planting management

[0094] A. The sowing time of the two places is late September 2017. Before sowing, plow the cultivated land. If the cultivated crops are flue-cured tobacco and other crops that have been infected by root-knot nematode pathogens, first remove the residual roots of the previous crops, and level the cultivated land, and then sow the seeds by broadcastin...

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Abstract

The invention relates to a method of quickly decomposing green straws of a plant and preventing and controlling root knot nematode disease of flue-cured tobacco. The method comprises the following steps: selecting oil radish with a root knot nematode disease resistant gene as a green straw of green manure; screening four microorganisms which have preventing and controlling effects and decompose the green straw efficiently to the root knot nematode disease; spraying a decomposing fungicide in a full-bloom stage by proportioning the four fungicides at proper concentrations; and turning over anddecomposing the green straw sprayed by the fungicide locally for over 60 days, wherein by accelerating quick and efficient decomposition of the green straw, in the condition of reducing 30% of a chemical fertilizer, organic matters of rhizosphere soil of flue-cured tobacco cultivation are degraded quickly, and primary nutrients: nitrogen phosphorus and potassium are full. The method is obvious inpreventing and controlling effects to the root knot nematode disease in the flue-cured tobacco cultivation process, and the tobacco leaf output value is improved obviously. The invention relates to the simple method which decomposes the green straw quickly, increases organic matters and nutrients of soil effectively, prevents and controls root knot nematode disease of cultivated crops in the nextstubble and improves the crop output and quality.

Description

technical field [0001] The invention belongs to the application field of plant protection and crop nutrition, and specifically relates to a method in which plant green straws are quickly decomposed into crop nutrients by fermenting bacterial agents in the field, and have a control effect on root-knot nematode disease. Background technique [0002] For a long time, in my country's agricultural production, the application of chemical fertilizers is usually used to increase the yield of crops, and the quality of crops is often ignored. Due to the long-term application of chemical fertilizers, the soil texture of cultivated crops has been degraded, and the non-point source pollution of water in agricultural production has caused a series of problems such as the deterioration of lake water quality and the sharp reduction of drinking water sources (Jinfeng, Green Technology, 2018). In recent years, all sectors of society have paid great attention to reducing chemical fertilizers a...

Claims

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Application Information

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IPC IPC(8): C05G3/02C05G3/04C05F17/00A01B79/02A01G13/00C12N1/20C12N1/14C12R1/10C12R1/125C12R1/885C12R1/685C05G3/60C05G3/80
CPCY02W30/40
Inventor 阙劲松张绍松黄坤余萍刘春明罗红梅陆庆华何建忠李宏光倪凤萍钟源杨启凡陈丽华邵小东冀新威普恩平付亚丽
Owner HONGHEZHOU BRANCH OF YUNNAN TOBACCO
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