Synthetic method of H&E-like image and optical system adopting same

Abstract

The invention discloses a synthetic method of an H&E-like image. The method comprises the following steps: inputting a gray scale interference image or a gray scale reflection image of a to-be-detected sample into a first storage block of an information processing device, and inputting a gray scale fluorescence image of the to-be-detected sample into a second storage block of the information processing device; converting the gray-scale interference image or the gray-scale reflection image into a first RGB image through a first color conversion operation by using the information processing device, and converting the gray-scale fluorescence image into a second RGB image through a second color conversion operation by using the information processing device; an image fusion operation and an intensity inversion operation are performed on the first RGB image and the second RGB image by using the information processing device to generate a class of H & E images. In addition, the invention also discloses an optical system adopting the method.

Description

technical field [0001] The invention relates to an image synthesis method of an optical system, in particular to an H&E (Hematoxylin and Eosin, hematoxylin and eosin) image synthesis method and an optical system using the method. Background technique [0002] When performing tumor resection, it is often necessary to wait for the pathologist to use a frozen section (frozen section) to test to determine whether the tumor is completely removed. However, in the process of frozen sectioning, if the sample to be tested is a sample with a lot of water, it will produce ice crystals (crystal ice) that destroy the tissue structure after freezing; and if the sample to be tested is a sample with a lot of fat (fat), At the temperature (~-20°C) where all tissues have been frozen and solidified, the adipose tissue will easily fall off from the slice because it has not been frozen and solidified, resulting in incomplete sliced ​​tissue. In addition, frozen cells are not easy to be stained ...

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Problems solved by technology

[0006] However, since the patent framework uses multiple fluorescence images to generate H&E-like images, it still has the following disadvantages: (1) The color contrast of this type of H&E images still needs to be improved because the structures in the sample to be tested cannot be fully dyed with fluorescence; (2) Various fluorescent agents needed to generate fluorescent images will still cause damage to the sample to be tested; (3) Dyeing with various fluorescent dyes takes a long time

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