The invention discloses a method for making paraffin slices of Xanthos sorbifolium cuttage branches and calluses thereof, comprising: taking materials and fixing them, washing, softening, dehydrating, transparentizing, dipping in wax and embedding, slicing, sticking and unfolding, removing Waxing and dyeing, and sealing; the specific method of washing is: repeatedly wash the stem section after getting the materials and fixing it three times with 50% ethanol; Hydrofluoric acid, glycerol with a mass fraction of 50% at 50° C. and a softening solution are soaked for a certain period of time; wherein, the softening solution is an equal volume mixture of alcohol with a mass fraction of 50% and glycerol with a mass fraction of 100%. The method of the present invention has the advantages of easy operation, good stability of results, convenience for a large number of basic researches, complete slices, uniform thickness, clear tissue structure, good dyeing contrast, complete cell shape, and the obtained slices of X. sorbifolium callus have good Continuity, can be preserved forever.