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A medicine-carrying chitosan/gelatin slow-release microparticle algistat and a preparing method thereof

A technology of slow-release microparticles and chitosan, applied in the fields of botanical equipment and methods, herbicides and algicides, sterilization/microdynamic water/sewage treatment, etc. The effect of good capacitance, cost reduction, and inhibition of algae outbreaks

Inactive Publication Date: 2019-05-21
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Slow release of drug-loaded microparticles is a technology to control the release rate and release direction of embedded drugs, and there are few researches and applications in the governance of water ecology.

Method used

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  • A medicine-carrying chitosan/gelatin slow-release microparticle algistat and a preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] S1. Weigh 0.1g chitosan and dissolve it in 10ml of 1% glacial acetic acid solution, and add 0.02g phenolic allelopathic active substances therein;

[0034] S2. Add the drug-loaded chitosan solution into 80ml of liquid paraffin containing 3.2ml span80, stir evenly at 50°C at a speed of 1000r / min, and emulsify for 15min;

[0035] S3, then drop 10ml of 20mg / ml gelatin aqueous solution into the above emulsion, continue to stir and emulsify at 50°C for 15min, then stir and cool to 15°C;

[0036] S4. Add 1.5ml of glutaraldehyde, stir and solidify for 60min; centrifuge at 800r / min for 10min, wash the precipitate with petroleum ether and absolute ethanol for 3 times, then suction filter, and finally vacuum-dry at 40°C to obtain the drug-loaded shell Glycan / gelatin sustained-release microparticles.

[0037] Acute toxicity test on aseptically cultured Microcystis aeruginosa using drug-loaded chitosan / gelatin sustained-release particulate algicide:

[0038] Set up three parallel...

Embodiment 2

[0040] S1. Take 0.2g chitosan and dissolve it in 20ml of 1% glacial acetic acid solution, and add 0.04g phenolic allelopathic active substances therein;

[0041] S2. Add the drug-loaded chitosan solution into 150ml of liquid paraffin containing 6mlspan80, stir evenly at a speed of 1000r / min at 50°C, and emulsify for 15min;

[0042] S3, then drop 30ml of 20mg / ml gelatin aqueous solution into the above emulsion, continue stirring and emulsifying at 50°C for 15min, then stir and cool to 15°C;

[0043] S4. Add 2.5ml of glutaraldehyde, stir and solidify for 60min; centrifuge at 800r / min for 10min, wash the precipitate with petroleum ether and absolute ethanol for 3 times, then suction filter, and finally vacuum-dry at 40°C to obtain the drug-loaded shell Glycan / gelatin sustained-release microparticles.

[0044] Acute toxicity test on aseptically cultured Microcystis aeruginosa using drug-loaded chitosan / gelatin sustained-release particulate algicide:

[0045] Set up three paralle...

Embodiment 3

[0047] S1. Take 0.15g chitosan and dissolve it in 15ml of 1% glacial acetic acid solution, and add 0.03g phenolic allelopathic active substances therein;

[0048] S2. Add the drug-loaded chitosan solution into 100ml of liquid paraffin containing 3.5mlspan80, stir evenly at 50°C at a speed of 800r / min, and emulsify for 15min;

[0049] S3, then drop 20ml of 20mg / ml gelatin aqueous solution into the above-mentioned emulsion, continue to stir and emulsify at 50°C for 15min, then stir and cool to 15°C;

[0050] S4. Add 2ml of glutaraldehyde, stir and solidify for 60min; centrifuge at 800r / min for 10min, wash the precipitate with petroleum ether and absolute ethanol for 3 times, then suction filter, and finally vacuum-dry at 40°C to obtain drug-loaded chitosan Sugar / gelatin extended-release microparticles.

[0051] Acute toxicity test on aseptically cultured Microcystis aeruginosa using drug-loaded chitosan / gelatin sustained-release particulate algicide:

[0052] Set up three para...

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Abstract

A medicine-carrying chitosan / gelatin slow-release microparticle algistat is disclosed. Chitosan and gelatin are adopted as carrier materials to embed the phenolic acid type active allelochemical through an emulsification crosslinking reaction to prepare the slow-release microparticles. Preparation steps include adding the allelochemical into a glacial acetic acid solution containing chitosan; adding the solution into liquid paraffin containing span80; performing uniform high-temperature emulsification; then adding an aqueous gelatin solution into the emulsion mentioned above, stirring and emulsifying the mixture, cooling the mixture, and then adding dropwise glutaraldehyde for crosslinking and curing; performing centrifugation, washing, precipitation, suction filtration and then vacuum drying to obtain the slow-release microparticles. The phenolic acid type active allelochemical and the micro-nano capsule technique are combined to prepare the slow-release microparticle algistat which is small in particles size and which is not liable to expand or sink. Microcystis aeruginosa suppressing time of the allelochemical in water bodies is prolonged, and the cost is reduced. The algistat is applied in real algal inhibition field, and provides a novel technical route for application of the phenolic acid type active allelochemical in algal inhibition in natural water bodies.

Description

technical field [0001] The invention relates to an algae inhibitor and a preparation method, in particular to a drug-loaded chitosan / gelatin slow-release particulate algae inhibitor and a preparation method, and belongs to the technical field of water body eutrophication inhibition of algae outbreak. Background technique [0002] Microcapsules have broad application prospects in the field of drug controlled release because of their excellent controlled release properties such as long-acting, high-efficiency, targeting, and low side effects. Because this technology is developed in interdisciplinary fields, it has become a research hotspot for workers in the fields of materials, chemistry, chemical engineering, biology and medicine at home and abroad. The sustained release of drug-loaded microparticles is a technology to control the release rate and release direction of embedded drugs, and there are few researches and applications in the treatment of aquatic ecology. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/28A01N37/40A01N31/16A01N37/38A01N31/08A01P13/02C02F1/50
Inventor 倪利晓顾国秀胡玲玲荣诗怡刘烜瑜岳菲菲杜存浩
Owner HOHAI UNIV
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