Unlock instant, AI-driven research and patent intelligence for your innovation.

Molecular marker R112823 in close linkage with rice blast resistance gene Pi1

A rice blast, molecular marker technology, applied in the field of molecular biology, can solve the problems of lack and complex genetic mechanism of rice blast resistance.

Inactive Publication Date: 2019-05-24
SHENZHEN INST OF BIOLOGICAL BREEDING & INNOVATION CHINESE ACADEMY OF AGRI SCI +2
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, although some rice blast resistance genes and disease resistance loci have been located, such as Pi-a, Pii, Pik, Pi1, etc., due to the complexity of the genetic mechanism of rice blast resistance, there is still a lack of sufficient numbers and loci. QTLs and molecular markers related to rice blast resistance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker R112823 in close linkage with rice blast resistance gene Pi1
  • Molecular marker R112823 in close linkage with rice blast resistance gene Pi1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Construction of rice F2 generation segregation population and collection of field phenotype data of F2 generation

[0040] The female parent is an indica two-line sterile line GD-7S, which exhibits broad-spectrum and high resistance to rice blast.

[0041] The male parent is an indica-type high-quality restorer line R-1128, which is a high-quality indica-type restorer line with thick stems, moderate plant type, straight leaves, high spike rate, large spikes and many grains, and good combining ability. Susceptible to rice blast.

[0042] The pollen of the male parent and the female parent were crossed to obtain F1, and the F1 generation was self-crossed to generate the F2 generation population, with a total of 264 individual plants. The F2 population was planted in the Shenzhen Huada Agricultural Experimental Base Park, and the rice blast resistance of the offspring was observed, and the phenotypic data were determined.

[0043] The phenotypic data showed that among...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a molecular marker R112823 in close linkage with the rice blast resistance gene Pi1. The molecular marker comprises sequences as shown as SEQ ID NO:1. The invention further relates to a primer for amplifying the molecular maker, application of the primer and the molecular maker in positioning of the rice blast resistance gene Pi1 or rice genetic breeding, and a rice breedingmethod. By the molecular maker, a relation between the genomic DNA sequences and the rice blast resistance gene Pi1 is built, which facilitates building of the molecular maker assisted rice breedingsystem; particularly, the molecular marker and the rice blast resistance gene Pi1 are in close linkage. The molecular marker and the amplification primer thereof can be applied to rice breeding practice and resource and variety identification in a simple, convenient, rapid and high-throughput manner.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a molecular marker, in particular to a molecular marker closely linked with rice blast resistance gene Pi1. The invention also relates to a primer for amplifying the molecular marker, and the use of the molecular marker and the primer in positioning rice blast resistance gene Pi1 or rice genetic breeding. Background technique [0002] Rice blast (Rice Blast) is one of the important diseases of rice. This disease can cause a large reduction in rice yield, and in severe cases, the yield can be reduced by 40% to 50%, or even crop failure. The control of rice blast plays a vital role in the guarantee of food security in my country. At present, planting disease-resistant varieties is the most economical and effective measure to control the disease, but there are few disease-resistant varieties in production, and many disease-resistant varieties lose their resistance to rice blast after ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11C12N15/82
Inventor 张耕耘侯军亮李刚蔡伟鄢阳天赵山岑崔居多
Owner SHENZHEN INST OF BIOLOGICAL BREEDING & INNOVATION CHINESE ACADEMY OF AGRI SCI