Compound, its preparation method and its fluorescent probe and application

Abstract

The invention discloses a compound I. The compound I has a structure shown in a formula I (the formula I is shown in the description), wherein R1 is an acyl group, phenyl group or alkyl oxycarbonyl group containing 2-5 carbon atoms; R2 is a halogen atom or hydrogen atom; R3 is an alkyl group containing 1-18 carbon atoms. The invention also discloses a synthesizing method of the compound I, a fluorescent probe containing the compound I and application. The fluorescent probe provided by the invention has the advantages that the selectivity is excellent, the reaction is rapid, and fibroblast activating protein alpha can be simply, rapidly and visually detected.

Description

technical field [0001] The application relates to a fluorescent probe and its preparation method and application, belonging to the field of small organic molecule probes. Background technique [0002] Fibroblast activating protein α (FAPα) is a type II transmembrane glycoprotein with 760 amino acids, which belongs to the serine protease family and has dipeptidyl peptidase and collagenase activities. Studies have confirmed that FAPα is specifically expressed in the matrix of malignant epithelial tumors including breast cancer, skin melanoma, colon cancer, pancreatic cancer, lung cancer, and ovarian cancer, while normal human tissues except embryos, healing wounds, and physiologically reconstructed organs , generally not expressed. Studies have shown that FAPα is widely involved in the processes of growth, invasion, metastasis, angiogenesis, remodeling of extra-tumor matrix, and anti-tumor immunity in tumorigenesis and development. [0003] Therefore, fibroblast activation p...

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Problems solved by technology

[0003] Therefore, fibroblast activation protein is expected to become a new effective target and play a huge role in the early detection and treatment of tumors. At present, although the drug research on FAPα inhibitors has led to the discovery of many FAPα-targeted inhibitors, including Candidate drugs in the second phase of clinical trials, but the current detection methods to determine whether FAPα is highly expressed in tissues are usually based on tissue sections, staining, and western blotting. The operation is more complicated and time-consuming

In the early days, there were not many related studies on rapid, simple and visual detection of FAPα. Some current reports on the detection of FAPα have short emission wavelengths of probes, and the application of in vivo imaging has certain limitations. In view of this, this application is proposed

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