Walnut protein polypeptide with ACE inhibiting activity and preparation method and application thereof

A technology of inhibiting activity and walnut meal is applied in the field of walnut meal polypeptide, and walnut meal is used as raw material to prepare the polypeptide, which can solve the problems of large side effects, short drug effect time, long-term use by patients, etc., and achieve good economic benefits, improvement and comprehensive utilization. horizontal effect

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the antihypertensive drugs currently used are chemically synthesized, such as captopril, ramipril, etc. These drugs have better antihypertensive effect, but the drug effect time is short, patients need to take it for a long time, and the side effects are relatively large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Preparation of walnut meal defatted powder

[0037] The walnut pulp after oil extraction is dried in a blast drying oven at 45°C, crushed, passed through a 60-mesh sieve, and then analytically pure petroleum ether with a boiling range of 30-60°C is added according to a material-liquid ratio of 1g:10mL to remove the remaining walnut oil to obtain walnut meal defatted powder.

[0038] 2. Extract walnut gluten-1

[0039] (1) According to the solid-liquid ratio of 1g:15mL, add deionized water to the degreased walnut meal powder, stir and extract at room temperature for 2.5h, filter, and collect the filtrate. Add deionized water (solid-liquid ratio: 1g:15mL) to the filter residue and extract repeatedly 3 times according to the above method, and combine the collected filtrates.

[0040] (2) Centrifuge the combined filtrate of step (1) at 4°C and 10,000rpm for 20min, then add 0.4mol / L sodium chloride aqueous solution to the obtained precipitate according to the solid-liqu...

Embodiment 2

[0052] 1. Preparation of walnut meal defatted powder

[0053] The walnut pulp after oil extraction is dried in a blast drying oven at 40°C, crushed, passed through a 80-mesh sieve, and then analytically pure petroleum ether with a boiling range of 30-60°C is added according to a material-to-liquid ratio of 1g:15mL to remove the remaining walnut oil to obtain walnut meal defatted powder.

[0054] 2. Extract walnut gluten-1

[0055] (1) According to the solid-liquid ratio of 1g:10mL, add deionized water to the degreased walnut meal powder, stir and extract at room temperature for 3 hours, filter, and collect the filtrate. Add deionized water (solid-liquid ratio: 1g:10mL) to the filter residue and extract repeatedly 3 times according to the above method, and combine the collected filtrates.

[0056] (2) Centrifuge the combined filtrate of step (1) at 5°C and 12000rpm for 15min, then add 0.5mol / L sodium chloride aqueous solution to the obtained precipitate according to the solid...

Embodiment 3

[0067] ACE inhibitory activity of walnut meal polypeptide of the present invention

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Abstract

The invention discloses walnut protein polypeptide with ACE inhibiting activity and a preparation method and application thereof. The polypeptide is a mixture of compounds with amino acid sequences ofVal-Glu-Arg-Gly-Arg-Arg-Ile-Thr-Ser-Val and Phe-Val-Ile-Glu-Pro-Asn-Ile-Thr-Pro-Ala. According to the preparation method of the walnut protein polypeptide with the ACE inhibiting activity, firstly, waste walnut protein which is obtained after oil processing is conducted is subjected to degreasing treatment, then a continuous extraction method is adopted, pure water, a diluted salt solution, an ethyl alcohol solution and a diluted acid solution are sequentially used for extracting walnut glutelin-1 from degreased walnut protein, then the walnut glutelin-1 is used as a raw material, and technologies of limited trypsinization, sephadex chromatographic separation, reserve efficient liquid-phase chromatography purification and the like are adopted for preparing the walnut protein polypeptide with an ACE activity inhibition ratio over 92%. According to the walnut protein polypeptide with the ACE inhibiting activity and the preparation method and the application thereof, the waste can be turned into wealth, the walnut deep-processing and comprehensive utilization levels are raised, development of the walnut industry is promoted, good economic benefits are generated, and the obtained polypeptide can be used as an ACE inhibitor and can be used for preparing an antihypertensive drug.

Description

technical field [0001] The invention belongs to the technical field of agricultural product processing, and in particular relates to a walnut meal polypeptide with ACE inhibitory activity and a method for preparing the polypeptide by using walnut meal as a raw material. Background technique [0002] Walnut (Juglans regia L.), also known as walnut and Qiang peach, is an important economically cultivated tree in my country. At present, there are 27 main varieties in my country, and the cultivation areas involve 26 provinces. The main production areas are Xinjiang, Shaanxi, Gansu, Yunnan, Shanxi and other provinces. According to the China Forestry Statistical Yearbook, the annual output of walnuts in my country reached 4.1525 million tons in 2017. Based on the price of 16 yuan / kg, the output value of walnuts in China reached 66.4 billion yuan in 2017. The walnut market in my country has broad prospects in the future. [0003] Studies have shown that the protein content in waln...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/36C07K1/30C07K1/34C12P21/06C07K1/20C07K1/16A61P9/12
Inventor 张有林王静张润光张玉峰王国良赵爱青
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