Cell bionic intelligent production system

A production system and cell technology, applied in the field of cell bionic intelligent production system, can solve the problems of adverse effects, low red blood cell denucleation efficiency, etc.

Active Publication Date: 2019-05-31
赵涌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are studies that can continuously expand hematopoietic stem cells in vitro for 45 days in a serum-free culture system, and the number of cells can reach 10 7 times, but the red blood cell denuclearization efficiency is low, which cannot meet the requirements
Although the complete denucleation of erythroid progenitor cells is achieved by the hematopoietic support of stromal cells, these stromal cells have adverse effects on later blood transfusions

Method used

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  • Cell bionic intelligent production system
  • Cell bionic intelligent production system
  • Cell bionic intelligent production system

Examples

Experimental program
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Effect test

preparation example Construction

[0042] The hollow fiber tube of the present invention can be prepared by a known method. In an exemplary method, the preparation method of the hollow fiber tube of the present invention includes the following steps:

[0043] (1') Steps for preparing activated hollow fiber

[0044] The activated hollow fiber can be prepared by methods known in the art. In some embodiments, the activated hollow fiber is prepared using chloromethyl ether-modified polysulfone as a raw material by, for example, a melt extrusion method.

[0045] In an exemplary method, the melt extrusion method of chloromethyl ether modified polysulfone is used to produce plastic products, and the melt is gradually cooled in an atmospheric environment. As the melt temperature decreases, the melt fluidity gradually Reduce and solidify into shape.

[0046] In another exemplary method, the activated hollow fiber having an asymmetric structure is prepared by, for example, a profiled spinneret molding method. Specifically, it...

Embodiment 1

[0072] This embodiment is the preparation of the hollow fiber tube 1.

[0073] 0.2kg of polysulfone hollow fiber C2011 (FiberCell Systems, Frederick, MD) was immersed in 1000ml of organic solvent consisting of chloromethyl ether and dioxyethane in a volume ratio of 1:10, and 8g of chlorinated Zinc. The polysulfone hollow fiber was reacted at 40°C for 12 hours. The organic solvent is intermittently stirred during the reaction to keep the reaction between the hollow fiber tube and the organic solvent uniform. Then, the hollow fiber tube after multiple activations was washed with deionized water. It was then dried at 60°C to obtain activated hollow fibers. The activated hollow fiber is soaked in a 25% TMA solution at room temperature for 45 hours to obtain the hollow fiber tube 1 of the present invention.

Embodiment 2

[0075] This embodiment is the preparation of the hollow fiber tube 2.

[0076] Dissolve chloromethyl ether modified polysulfone in DMF organic solvent, use PEG6000 as an additive, and defoam by miscibility, using a metering pump (specification of 1.2ml / r), plug-in tube spinneret, nitrogen cylinder, etc. The hollow fiber membrane spinning machine prepares hollow fibers, and after exposure to air, a coagulation bath is carried out (the temperature of the gel bath is 25°C, the solvent concentration in the gel bath is 55%, and the solvent concentration in the core liquid is 78%). Thus, an activated hollow fiber tube having an asymmetric structure was prepared. The activated hollow fiber is soaked in a 25% TMA solution at room temperature for 45 hours to obtain the hollow fiber tube 2 of the present invention.

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Abstract

The invention discloses a cell bionic intelligent production system comprising a bioreactor, a medium container and an oxygen supply. The bioreactor comprises a sealed chamber formed by an outer casing and a plurality of hollow fiber tubes disposed in the chamber. The outer casing is provided with a medium inlet, a medium outlet and an oxygen inlet. The inner cavity of each hollow fiber tube is capable of circulating a basic medium and / or gas, the tail end of each hollow fiber tube is communicated with the basic medium container and / or the oxygen supply, the wall of each hollow fiber tube allows the composition of the basic medium and / or the gas to pass through the tube wall from the inner cavity side of the hollow fiber tube to the outside, and at least the outer surface of the tube wallof each hollow fiber tube is provided with a group suitable for cell denucleation. The medium container is provided with a supply port, and the supply port is connected to a medium inlet through a pipe. The oxygen supply is connected to an oxygen inlet through a pipe. The cell bionic intelligent production system is particularly suitable for cultivation of erythroid progenitor cells.

Description

Technical field [0001] The invention relates to a cell production system, in particular to a cell bionic intelligent production system. Background technique [0002] Data from the World Health Organization (WHO) Global Blood Safety Database shows that 92 million units of whole blood donations are collected globally every year. Every day, 3.3% of hospitals postpone surgery due to blood shortages, and 10.3% of hospitals have at least 1 day of emergency surgery every year. Blood is available. In addition, 5-10% of blood transfusion is unsafe due to HIV infection and other reasons. Therefore, the demand for blood supply is very urgent on a global scale. [0003] With the in-depth research on the differentiation and development of hematopoietic stem cells, in vitro artificial induction of hematopoietic stem progenitor cells to expand and produce mature red blood cells has gradually become a potential and feasible direction to solve the difficulties faced by blood transfusion. At pres...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12M3/00C12M1/24C12M1/04C12M1/00
CPCA61M1/34C12M1/00C12M1/04C12M1/24C12M3/00
Inventor赵涌
Owner赵涌