Composition capable of catalyzing the degradation of uric acid in intestinal tract, its preparation method and application
A composition, technology of uric acid, applied in the field of degradation of uric acid or urate
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Embodiment 1
[0029] Embodiment 1: Isolation and preservation of Cordyceps militaris (Cordyceps militaris)
[0030] The researcher of the present invention buys fresh Cordyceps militaris from Wuhan farmer's market, adopts tissue separation method to carry out the separation and purification of Cordyceps militaris. The specific operation is as follows: clean the surface of fresh Cordyceps militaris fruiting body with sterile water, put it into a sterile petri dish in the ultra-clean workbench, clamp 75% (v / v) alcohol cotton balls on Cordyceps militaris with sterilized tweezers The surface of the fruiting body is disinfected, the fruiting body is cut open with a sterilized scalpel, and the bacterial meat tissue pieces of about 2*2mm inside the fruiting body are cut, moved to sterilized PDA slant medium (200g potato, 10g glucose , 15g of agarose, distilled to 1L with pure water, sterilized at 121°C), placed in a constant temperature incubator at 26°C for cultivation. Check the colony growth e...
Embodiment 2
[0031] Embodiment 2: the preparation method of the Cordyceps militaris fungus powder containing uric acid oxidase
[0032] The Cordyceps militaris (Cordyceps militaris) preserved in Example 1 on the PDA solid medium was picked and transferred to the fungal culture medium plate (peptone 0.5wt%, yeast extract powder 0.2wt%, glucose 2wt%, dihydrogen phosphate Potassium 0.1wt%, magnesium sulfate 0.05wt%, 2.0wt% agarose, pure water to make up to a constant volume, pH 6.2~6.6), culture at 25-28°C for 7-10 days for activation culture, cut 1cm×1cm from the plate The bacterial block, after chopping, is inoculated to the seed medium (sucrose 2.0wt%, peptone 1.0wt%, yeast extract 1.0wt%, dipotassium hydrogen phosphate 0.5wt%, sodium dihydrogen phosphate 1.0wt%, pure water constant volume Filling, pH6.5), placed at 150rpm, cultured at 25-28°C for 3-5 days, then crushed the bacterial block with a sterile hand-held homogenizer, continued to cultivate under the same conditions for 3-5 days, ...
Embodiment 3
[0033] Embodiment 3: the preparation method of the Cordyceps militaris fungus powder containing uric acid oxidase
[0034] The Cordyceps militaris (Cordyceps militaris) preserved in Example 1 on the PDA solid medium was picked and transferred to the fungal culture medium plate (peptone 0.5wt%, yeast extract powder 0.2wt%, glucose 2wt%, dihydrogen phosphate Potassium 0.1wt%, magnesium sulfate 0.05wt%, 2.0wt% agarose, pure water to fill up, pH 6.2 ~ 6.6), 25-28 ° C for 6-7 days for activation culture, cut from the plate 1cm × 1cm The bacterium block, after chopping, is inoculated to the seed culture medium (sucrose 2.0wt%, peptone 1.0wt%, yeast extract 1.0wt%, dipotassium hydrogen phosphate 1.0wt%, sodium dihydrogen phosphate 1.0wt%, pure water constant volume Filling, pH6.5), placed at 150rpm, cultured at 25-28°C for 3-5 days, then crushed the bacterial block with a sterile hand-held homogenizer, continued to cultivate under the same conditions for 3-5 days, and then used A st...
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