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Therapeutic sall4 peptide

一种中性、非极性的技术，应用在表达,4的,分离的肽接领域，能够解决缺乏靶标特异性等问题

Active Publication Date: 2019-06-07
NAT UNIV OF SINGAPORE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main limitation of this peptide-based therapy is the lack of target specificity

Method used

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  • Therapeutic sall4 peptide
  • Therapeutic sall4 peptide
  • Therapeutic sall4 peptide

Examples

Experimental program
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Effect test

Embodiment

[0085] SALL4 binds to RBBp4 of NuRD

[0086] It was previously reported that SALL4WT binds to the NURD complex and blocks the SALL4-NuRD interaction. The histone-binding protein RBBp4 was hypothesized to be the SALL4-binding subunit in NuRD. To confirm the direct binding of SALL4 to RBBp4, a binding assay was performed using the 12 amino acid WT SALL4T (Figure 1). Using isothermal titration calorimetry, a direct interaction between SALL4 and the RBBp4 subunit of the NURD complex was demonstrated. The 12-aa SALL4 peptide binds to the K of RBBp4 D 1.04±0.06μM ( Figure 1A ). The binding kinetics were further confirmed using surface plasmon resonance, where the binding K between the SALL4 peptide and RBBp4 D Calculated to be 1.5 μM (k on =16830±460M -1 the s -1 ;k off=0.026±0.00045s -1 )( Figure 1B ). These results demonstrate that SALL4 directly binds to the NuRD complex through the NuRD subunit RBBp4.

[0087] Crystal structure of the RBBp4-SALL4 complex

[0088]...

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Abstract

The present invention provides, in certain embodiments, isolated peptides and pharmaceutical compositions comprising isolated peptides that bind to retinoblastoma binding protein 4 RBBp4 such that, the binding blocks the SALL4-RBBp4 interaction. Methods of inhibiting binding of SALL4 with RBBp4 and methods for treating a subject having a disorder mediated by a dysregulation of SALL4 are also provided.

Description

[0001] related application [0002] This application claims the benefit of US Provisional Application 62 / 329,010, filed April 28, 2016. The entire teaching of this application is incorporated herein by reference. [0003] Cite material incorporated into an ASCII text file [0004] This application incorporates by reference the Sequence Listing contained in the following ASCII text file filed concurrently herewith: [0005] a) File name: 44591138001_SEQUENCELISTING.txt, created on April 28, 2017, 13KB in size. Background technique [0006] Spalt-like transcription factor 4 (SALL4) has an integral role in developmental events and maintenance of stem cell pluripotency. SALL4 is a zinc finger transcription factor that, together with POU5FI (Oct4), Nanog and Sox2, forms a core transcriptional network that activates genes associated with the proliferation of embryonic stem cells (ESCs). SALL4 binds to retinoblastoma-binding protein 4 (RBBp4, which is a subunit of the nucleosome ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/04A61K38/10A61K38/17C07K7/00C07K7/02C07K7/04C07K7/06
CPCA61K38/00C07K7/06C07K7/08C07K2319/033
Inventor 刘美慧D·G·特恩柴立谢青山A·波尔森
Owner NAT UNIV OF SINGAPORE
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