Method for extracting termitomyces albuminosa polysaccharide from termitomyces albuminosa root waste and application thereof
A technology of Galliconia spp. and waste, which can be used in drug combination, metabolic diseases, etc., can solve problems such as waste of resources, and achieve the effects of preventing decomposition, high extraction efficiency, and improving extraction rate.
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Embodiment 1
[0026] The method for extracting the polysaccharide from the root waste of Mycorrhizae collurea, comprising:
[0027] S1, removing impurities from the wastes from the roots of Mycorrhizae miltiorrhiza by vibrating sorting and sieving, and micronizing the waste from the roots of Mycorrhizae miltiorrhiza after the removal of impurities by a superfine pulverization method, and the final particle size of the finished product is below 10 μm;
[0028] S2, the ratio of material to liquid is 1:10 when mixing the root waste micropowder and deionized water, put the mixture into an ultrasonic extraction tank, control the extraction temperature to 28°C, the frequency to 35kHz, the intensity to 600W, and the extraction time After the extraction is completed, put the extract into a centrifuge, the centrifugation temperature is 15°C, the rotation speed is 7000 rpm, and the centrifugation time is 15 minutes. After centrifugation, the supernatant is left to remove the sediment; The ultrasonic ...
Embodiment 2
[0035] The method for extracting the polysaccharide from the root waste of Mycorrhizae collurea, comprising:
[0036] S1, removing impurities from the wastes from the roots of Mycorrhizae miltiorrhiza by vibrating sorting and sieving, and micronizing the waste from the roots of Mycorrhizae miltiorrhiza after the removal of impurities by a superfine pulverization method, and the final particle size of the finished product is below 10 μm;
[0037] S2, the ratio of material to liquid is 1:10 when mixing the root waste micropowder and deionized water, put the mixture into an ultrasonic extraction tank, control the extraction temperature to 28°C, the frequency to 35kHz, the intensity to 600W, and the extraction time After the extraction is completed, put the extract into a centrifuge, the centrifugation temperature is 15°C, the rotation speed is 7000 rpm, and the centrifugation time is 15 minutes. After centrifugation, the supernatant is left to remove the sediment; The ultrasonic ...
Embodiment 3
[0050] S1, removing impurities from the wastes from the roots of Mycorrhizae miltiorrhiza by vibrating sorting and sieving, and micronizing the waste from the roots of Mycorrhizae miltiorrhiza after the removal of impurities by a superfine pulverization method, and the final particle size of the finished product is below 10 μm;
[0051] S2, mix the root waste micropowder and deionized water at a ratio of 1:15, put the mixture into an ultrasonic extraction tank, control the extraction temperature to 30°C, the frequency to 35kHz, the intensity to 600W, and the extraction time After the extraction is completed, put the extract into a centrifuge, the centrifugation temperature is 15°C, the rotation speed is 7000 rpm, and the centrifugation time is 15 minutes. After centrifugation, the supernatant is left to remove the sediment; The ultrasonic extraction temperature was raised to 50°C, the frequency was set to 55kHz, the intensity was 900W, and the extraction time was 45min. After t...
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