Test method for content of reducing sugar in ionic liquid system

An ionic liquid and test method technology, applied in the field of reducing sugar content testing, can solve the problems of immature reducing sugar content test methods, human error in end point determination, and difficult operation, and achieve less sample consumption, improved accuracy, and extended good effect

Active Publication Date: 2019-06-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the prior art, the HPLC method only needs to dissolve the sample with a mobile phase, filter it through a 0.45 μm microporous membrane, and then inject the sample into a high-performance liquid chromatograph, which is easy to operate and has high sensitivity. , most chromatographic columns do not allow ionic liquids to enter, so they have great application limitations; the iodometric method, potassium permanganate method, and Fehling's reagent method are all based on the color change of the indicator to indicate the titration end point, and the end point judgment has great potential. Human error, and the operation of titrating while heating is difficult, resulting in low accuracy of these methods
In addition, the determination of reducing sugar content is mostly based on aqueous solution system, and the test method of reducing sugar content in ionic liquid system is still immature, and there are few studies involving the influence of ionic liquid on reducing sugar test system

Method used

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  • Test method for content of reducing sugar in ionic liquid system
  • Test method for content of reducing sugar in ionic liquid system
  • Test method for content of reducing sugar in ionic liquid system

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0062] Provide a kind of test method of reducing sugar content in 1-ethyl-3-methylimidazole acetate ([EMIM]OAc) system in the present embodiment, described test method comprises the following steps:

[0063] (1) Prepare [EMIM]OAc standard solution with gradient concentration: Accurately weigh 50.00g of [EMIM]OAc, add a small amount of deionized water to dissolve, transfer to a 100.00mL volumetric flask, and dilute to 100.00mL with deionized water, Obtain the [EMIM]OAc aqueous solution that concentration is 500.00g / L; Then take 5 colorimetric tubes, add respectively the described [EMIM]OAc aqueous solution of 0.50mL, 0.75mL, 1.00mL, 1.50mL, 2.00mL, further dilute with water to 2.00mL; add 0.60mL 5g / L DNS reagent to the above 5 groups of solutions respectively, shake well, put it in a boiling water bath for 5min, take it out, cool to room temperature with cold water, and dilute to 25.00mL to obtain [EMIM] with gradient concentration OAc standard solution.

[0064] Test the abso...

Embodiment 2

[0077] The difference between this embodiment and Example 1 is that the preparation of [EMIM]OAc recovery solution in step (4): mix 25mg glucose with 50g ionic liquid [EMIM]OAc uniformly to obtain [EMIM]OAc recovery solution, the [ The theoretical mass percentage of reducing sugar in the EMIM]OAc recovery solution is 0.05%.

[0078] Prepare the [EMIM]OAc recovery solution to a concentration of C I = 12.83g / L solution to be tested: Take 321mg [EMIM]OAc recovered solution and add it to the colorimetric tube, add water to dilute to 2mL, then add 0.60mL 5g / LDNS reagent, shake well, place in boiling water bath for 5min; Cool to room temperature with cold water, and then adjust the volume to 25.00 mL to obtain a solution to be tested with a [EMIM]OAc concentration of 12.83 g / L.

[0079] Test the absorbance of the solution to be tested with a UV-visible spectrophotometer, and the solution to be tested is at a wavelength λ I = Absorbance at 522nm is A S =0.179; put A S =0.179 is s...

Embodiment 3

[0083] A kind of 1,5-diazabicyclo[4.3.0]-5-nonene phosphate diethyl ester ([DBNE]DEP) system provides a method for testing the reducing sugar content in the present embodiment, the test method includes the following step:

[0084] (1) Prepare [DBNE]DEP standard solution with gradient concentration: Accurately weigh 50.00g of [DBNE]DEP, add a small amount of deionized water to dissolve, transfer to a 100.00mL volumetric flask, and dilute to 100.00mL with deionized water, Obtain the [DBNE]DEP aqueous solution that concentration is 500.00g / L; Then take 8 colorimetric tubes, add 0.25mL, 0.50mL, 0.75mL, 1.00mL, 1.25mL, 1.50mL, 1.75mL, 2.00mL of the said [DBNE] DEP aqueous solution, further dilute to 2.00mL with water; add 0.60mL 5g / L DNS reagent to the above 8 groups of solutions respectively, shake well, put it in a boiling water bath for 5min, take it out, cool it to room temperature with cold water, and set the volume to 25.00 mL to obtain a gradient concentration of [DBNE]DEP ...

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Abstract

The present invention provides a test method for content of reducing sugar in an ionic liquid system. The method comprises the following steps of: (1) establishing an unitary linear regression equation of the absorbance and the ionic liquid concentration; (2) determining the optimal ionic liquid concentration CI of noninterference reducing sugar test; (3) establishing an unitary linear regressionequation of the absorbance and the glucose concentration; (4) testing the absorbance when the concentration of the ionic liquid to be tested is CI, and substituting the absorbance into the equation obtained in the step (3) to calculate the concentration CS of the reducing sugar; and (5) making the weight percentage content of the reducing sugar in the ionic liquid to be tested [Omega] (%)=CS/CI*100%. The test method is suitable for various ionic liquid systems, is wide in application range and high in analysis result accuracy, does not need separation of the reducing sugar in the ionic liquid,is simple and fast in operation and high in detection sensitivity, and has important guiding significance for recycle of the ionic liquid and the separation of the reducing sugar.

Description

technical field [0001] The invention belongs to the technical field of sugar quantitative analysis, in particular to a method for testing reducing sugar content. Background technique [0002] Cellulose has a wide range of sources and abundant reserves, and regenerated fibers can be prepared through further dissolution, regeneration, processing, etc., and are widely used in chemical, textile, food, medicine, and biological materials. Ionic liquids have excellent physical and chemical properties such as high stability, good solubility, and adjustable structural properties. They can efficiently dissolve cellulose and prepare regenerated fibers with excellent properties. The process of dissolving cellulose with ionic liquid to prepare regenerated fiber has the advantages of controllable solubility, clean and pollution-free, and simple process route. However, the price of ionic liquid is high, and a large amount of ionic liquid aqueous solution is produced in the process of diss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N1/28
Inventor 张锁江康召青聂毅周乐潘凤娇王均凤
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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