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Method for screening cell biological effects of compound salvia miltiorrhiza tablets

A technology of compound Danshen tablets and cell biology, which is applied in compound screening, biochemical equipment and methods, drug screening, etc., and can solve problems such as difficult operation methods and inaccurate information

Pending Publication Date: 2020-12-04
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a screening method for the cell biological effect of Compound Danshen Tablets, so as to solve the problem that the information obtained by the existing screening and observation method proposed in the above-mentioned background technology is inaccurate and the operation mode is relatively difficult

Method used

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  • Method for screening cell biological effects of compound salvia miltiorrhiza tablets
  • Method for screening cell biological effects of compound salvia miltiorrhiza tablets
  • Method for screening cell biological effects of compound salvia miltiorrhiza tablets

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Experimental program
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Effect test

Embodiment

[0040] see Figure 1-7 , the present invention provides a technical solution:

[0041] 1.1 Experimental animals

[0042] Healthy adult SD rats, male, with a body weight of 200-240 g.

[0043] 1.2 Reagents

[0044] CCK-8 solution (Japan Tongjin Institute of Chemistry);

[0045] Penicillin streptomycin (Gibco);

[0046] Fetal bovine serum (FBS, Zhejiang Tianhang Biotechnology Co., Ltd.);

[0047] Phosphate buffer saline (PBS, Gibco company);

[0048] Trypsin solution (Gibco company);

[0049] Chloral hydrate (Sinopharm Chemical Reagent Co., Ltd., analytically pure);

[0050] RTCA plate (Hangzhou Aisen Biological Co., Ltd.);

[0051] DMEM medium (Corning company);

[0052] Compound Danshen Tablets (Guangxi Medicinal Botanical Garden Pharmaceutical Factory, batch number 171101-049);

[0053] Rat cardiomyocytes (H9C2 cells) and human umbilical vein endothelial cells (Huvec cells) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences;

[0054] Rat th...

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Abstract

The invention belongs to the technical field of compound salvia miltiorrhiza experiments, and particularly relates to a method for screening cell biological effects of compound salvia miltiorrhiza tablets. The method for screening the cell biological effects of the compound salvia miltiorrhiza tablets comprises the following specific steps: S1, sample concentration screening and cell strain screening: screening of proper drug mass concentration of action cells of the compound salvia miltiorrhiza tablets by a CCK-8 method: selection of experimental cells, cell culture, preparation of a test sample of the compound salvia miltiorrhiza tablets, cell plating and administration. The effective concentration range of cells is screened out by the CCK-8 method, cell strains specifically dependent onthe compound salvia miltiorrhiza tablets are screened out by an RTCA technology, methodological investigation is carried out, so that the optimal range of the effective concentration and observationtime of an RTCA experiment is obtained, and a foundation is laid for establishing cell index dissolution kinetics.

Description

technical field [0001] The invention relates to the field of compound salvia miltiorrhiza experimental technology, in particular to a method for screening the cell biological effects of compound salvia miltiorrhiza tablets. Background technique [0002] Due to the complex composition of traditional Chinese medicine compound solid preparations, which have the characteristics of multi-components, multi-targets and overall effects, the properties of the multi-components of the compound and their behaviors in vivo and in vitro are different, and the release and dissolution mechanisms are inconsistent. [0003] It is necessary to observe the changes of different physiological states of cells. The existing screening and observation methods obtain incomplete information, and the operation method is relatively difficult. Contents of the invention [0004] The purpose of the present invention is to provide a screening method for the cell biological effect of Compound Danshen Tablet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCG01N33/5061G01N33/5064C12N2503/02G01N2500/10
Inventor 严国俊马晓斐顾薇陈军周悦马丽霞余亦婷张倩庄欣雅
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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