A kind of Hericium ergothioneine health product preparation and preparation method thereof
A technology of ergothioneine and Hericium erinaceus, applied in botany equipment and methods, applications, gardening, etc., can solve the problems of long cycle, bacterial contamination, waste, etc.
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Embodiment 1
[0090] (1) The strains preserved in test tubes were inoculated into 500 ml of seed culture medium with bacterial blocks, and cultured at 25° C. for 7 days on a shaker at 200 rpm;
[0091]The composition of the seed medium is as follows:
[0092] Glucose 20g / L, yeast powder 20g / L, disodium hydrogen phosphate 0.5g / L, acetic acid to adjust pH to 6.5
[0093] (2) Seed culture medium was inoculated into 15L fermentation medium, 200rpm shaker, cultured at 25°C for 11 days until residual sugar <0.5% fermentation ended;
[0094] The composition of the fermentation medium is as follows:
[0095] Glucose 60g / L, yeast powder 35g / L, sodium sulfate 0.75g / L, disodium hydrogen phosphate 1g / L, zinc chloride 0.005g / L, pyridoxal phosphate 0.001g / L, acetic acid to adjust pH to 5.5, asparagus Amino acid 5mM, glutamine 5mM, histidine, 5mM, betaine 20Mm.
Embodiment 2
[0097] (1) Take 500ml of the fermented liquid that has been fermented in Example 1, homogenize it through a high-pressure homogenizer, the homogenate pressure is 35MPa, and the homogenate time is 20min, add 25g of maltodextrin to the fermented liquid, heat to 50°C and stir Mix well to fully dissolve the excipients;
[0098] (2) The feed liquid is spray-dried, using pressure spraying, the feed rate is 400ml / h, the inlet air temperature is 180°C, and the outlet air temperature is 85°C. Finally, 42g of brown-yellow Hericium erinaceus powder can be obtained, and the liquid phase detection The content of ergothioneine in the bacterial powder was 0.222g / kg.
Embodiment 3
[0100] (1) Get 10L of fermented liquid that fermented in Example 1 and finish, homogenate through high-pressure homogenizer, homogenate pressure is 50MPa, homogenate time is 30min, after homogenization finishes, add maltodextrin 1500g in fermented liquid, heat Stir at 60°C to fully dissolve the excipients;
[0101] (2) The feed liquid is spray-dried, using centrifugal spraying, the feed rate is 2000ml / h, the inlet air temperature is 190°C, and the outlet air temperature is 95°C, finally 2.36kg of light yellow Hericium erinaceus powder can be obtained, liquid phase The content of ergothioneine in the bacterial powder was detected to be 0.146g / kg.
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