Preparation method of novel estrogen receptor protein ERbetaX and application thereof
A technology of estrogen receptor and protein, which is applied in the field of biomedicine, can solve the problems of large protein molecular weight, low ligand affinity, and low transfection efficiency, and achieve significant social effects, improve life quality, and have less toxic and side effects
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[0021] The present invention will be further described below in conjunction with accompanying drawing:
[0022] Such as figure 1 Shown, method of the present invention comprises the following steps:
[0023] S1, the preparation of ERβX protein by molecular biology technology; separation and purification by molecular sieve gel chromatography, ion exchange resin and wah gel chromatography; identification and structural analysis by liquid chromatography-mass spectrometer, amino acid sequence analyzer or nuclear magnetic resonance instrument;
[0024] S2, establish animal models of mice and rats, and conduct toxicology experiments of ERβX; clarify the dosage and route of ERβX;
[0025] S3, ERβX protein activity and pharmacokinetics research by high performance liquid chromatography (HPLC); biochemical analysis of specificity and affinity between ERβX and ligands such as estradiol (E2) and tamoxicol (TAM) characteristic;
[0026] S4, biological and molecular biological experimen...
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