Preparation method of novel estrogen receptor protein ERbetaX and application thereof

A technology of estrogen receptor and protein, which is applied in the field of biomedicine, can solve the problems of large protein molecular weight, low ligand affinity, and low transfection efficiency, and achieve significant social effects, improve life quality, and have less toxic and side effects

Inactive Publication Date: 2019-06-28
JILIN UNIV
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Problems solved by technology

The market needs to develop a new type of anti-cancer drug with small molecular weight, high efficiency and specificity to solve the problems of large molecular weight of common estrogen receptor protein, low transfection efficiency, poor selectivity and low ligand affinity

Method used

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  • Preparation method of novel estrogen receptor protein ERbetaX and application thereof

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with accompanying drawing:

[0022] Such as figure 1 Shown, method of the present invention comprises the following steps:

[0023] S1, the preparation of ERβX protein by molecular biology technology; separation and purification by molecular sieve gel chromatography, ion exchange resin and wah gel chromatography; identification and structural analysis by liquid chromatography-mass spectrometer, amino acid sequence analyzer or nuclear magnetic resonance instrument;

[0024] S2, establish animal models of mice and rats, and conduct toxicology experiments of ERβX; clarify the dosage and route of ERβX;

[0025] S3, ERβX protein activity and pharmacokinetics research by high performance liquid chromatography (HPLC); biochemical analysis of specificity and affinity between ERβX and ligands such as estradiol (E2) and tamoxicol (TAM) characteristic;

[0026] S4, biological and molecular biological experimen...

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Abstract

The invention discloses a preparation method of a novel estrogen receptor protein ERbetaX and an application of the ERbetaX protein, and which prepares ERbetaX protein by using molecular biological techniques; separating and purifying by adopting molecular sieve gel chromatography, ion exchange resin and silica gel chromatography; identifying and analyzing the structure through a liquid chromatography-mass spectrometry instrument, an amino acid sequence analyzer or a nuclear magnetic resonance instrument; establishing animal models of mice and rats, and carrying out toxicological experiments of ERbetaX; determining the administration dosage and the route of ERbetaX; carrying out research on the activity and pharmacokinetics of ERbetaX protein by high performance liquid chromatography (HPLC); analyzing the specificity and affinity of ligands such as estradiol (E2), Tamoxifen (TAM) and the like; studying the role and mechanism of ERbetaX in various hormone-related tumors such as breast cancer, prostate cancer, cervical cancer, ovarian cancer and colorectal cancer by biological and molecular biology experiments. The preparation method of the novel estrogen receptor protein ERbetaX hassmall toxic and side effects and low production cost, and avoids pain caused by radiotherapy and chemotherapy of patients due to surgery.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method and application of a novel estrogen receptor protein ERβX. Background technique [0002] Estrogen is one of the most important steroid hormones with a wide range of effects in the human body, and it exerts a wide range of biological effects mainly through the estrogen receptor (ER). As a steroid hormone, it mainly mediates the activation and dimerization of ER through the classic ligand-receptor binding mode, promotes its entry into the nucleus, and acts as a transcription factor to regulate the transcription activation process of target genes. After estrogen binds to ER, it can directly bind to the estrogen response element (ERE) on the target gene promoter to promote the transcriptional activation of the target gene; it can also interact with other transcription factors, such as activated protein 1 (AP1), specific protein 1 (SP1), nuclear factor-кB (NF-кB), cAMP...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/72C07K1/18C07K1/16C12N15/12C12N15/85A61K49/00
Inventor 李峰赵丽晶郭丽荣熊文激
Owner JILIN UNIV
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