Portable spectrum detecting device and method based on laser disc

Abstract

The invention discloses a portable spectrum detecting device and method based on a laser disc. In the device, a CD sheet is used to construct an exquisite spectrum detection structure. The method comprises the following steps of firstly, through irradiation of lasers with different wavelengths, collecting images through using an intelligent mobile phone, selecting an optimal pixel collection pointand establishing a conversion relationship between a vertical position and a wavelength; then, adding solutions with different concentrations, making a surface light source being transmitted througha cuvette and making the light source enter into a CD chamber through a slit, diffracting and imaging on a mobile phone camera through the CD sheet, using the intelligent mobile phone to collect images, obtaining a value of a V component in a HSV space of a corresponding position of an emission wavelength, and fitting a calibration curve of the solution concentration through a least square method;and substituting the value of the V component of the corresponding position in the image of a liquid to be tested into the calibration curve so as to calculate the concentration. In the invention, spectrum detection and quantitative measurement of a chromogenic reaction solution are realized, the device and the method have advantages of low cost, simple operation, rapid detection and the like, and simultaneously a demand of site detection can be satisfied.

Description

technical field [0001] The present invention relates to a portable spectrum detection device and method, in particular to a portable spectrum detection device and method based on a laser disc (CD). Background technique [0002] Nowadays, more and more research teams are committed to transforming biomolecular detection methods based on laboratory instruments into mobile platform-based detection methods for rapid, instant, on-site detection and detection of pathogens, biotoxins, heavy metals and other environmental pollutants. analyze. With the use of mobile phones becoming more and more popular, the performance of mobile phones is also improving rapidly, especially the rapid development of mobile phone cameras, making it possible to capture images with high resolution and high adaptability. Therefore, many scientific researchers use the built-in camera of the smartphone as an optical sensor to monitor fluorescent microspheres, detect liquid color changes in enzyme-linked imm...

AI Technical Summary

Problems solved by technology

At present, the detection and analysis of spectra usually use microplate readers, which are bulky, expensive and require professional personnel to operate, which cannot meet the needs of on-site detection

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