Pine tree endophytic bacterium FBS135 and application thereof

A FBS135, endophytic bacteria technology, applied in the field of microorganisms, can solve the problem of few endophytes, and achieve the effects of increasing system stress resistance, promoting growth indicators, and reducing infection

Active Publication Date: 2019-07-09
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To date, studies on endophytes have mainly focused on crops, while little has been done on endophytes in woody plants

Method used

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  • Pine tree endophytic bacterium FBS135 and application thereof
  • Pine tree endophytic bacterium FBS135 and application thereof
  • Pine tree endophytic bacterium FBS135 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation and identification of pine endophytic bacteria FBS135:

[0024] The terminal buds and pine needles were all obtained from Pine massoniana growing on the campus of Nanjing Forestry University, and the terminal buds and pine needles were subjected to strict surface disinfection (first, the tissue was washed with sterile water for 3 minutes, and then the tissue was washed in 75% ethanol solution Soak in 5% sodium hypochlorite solution for 1 minute, then transfer it to 5% sodium hypochlorite solution and soak for 5 minutes, all steps are carried out under constant shaking), then rinse the surface-sterilized tissue with sterile deionized water 5 times, in order to ensure The sample surfaces were completely sterilized, and a final rinse was applied to the LB agar and incubated overnight at 30°C to check for any microbial growth. Put the treated tissue into a sterile Petri dish, cut it into 2mm thick thin slices with an autoclaved blade, attach it to the LB medium, a...

Embodiment 2

[0028] Antibiotic Resistance Testing:

[0029] Antibiotics commonly used in the laboratory were used to detect their inhibitory effect on FBS135: chloramphenicol (Cm) was 5 μg / mL, ampicillin (Amp) was 100 μg / mL, erythromycin (Em) was 5 μg / mL, kanamycin (Km) was 5 μg / mL, spectinomycin (Spe) was 100 μg / mL. Cultivate FBS135 in LB medium to the logarithmic growth phase, and then spread evenly on LB solid medium containing one of the above-mentioned antibiotics. After incubating overnight at 30°C, observe and record the growth of FBS135.

[0030] Antibiotic tests showed that the resistance of FBS135 to erythromycin was 5 μg / mL, but to chloramphenicol (5 μg / ml), ampicillin (100 μg / ml), kanamycin (5 μg / mL) and spectinomycin ( 100μg / ml) sensitive.

Embodiment 3

[0032]Effect test of FBS135 on the growth of Pinus massoniana seedlings:

[0033] Experimental method: Take soil samples from Beida Mountain on the campus of Nanjing Forestry University, mix them well and place them in flowerpots with a diameter of 15cm. Select 30 annual masson pine seedlings with similar size and similar growth state, cut the nutrient bag and plant in the above-mentioned flower pots, and each pine seedling is used in an independent flower pot. The planted seedlings were randomly divided into 3 groups with 10 plants in each group. Use LB medium to shake FBS135 at 30°C to OD 600 Be 1, prepare pine tree growth promoter, for the 10 seedlings of the first group (irrigation group), slowly irrigate 30ml pine tree growth promoter into the soil that is 3cm around the seedling center radius; For the second group (spray group) For 10 seedlings, 20ml of the pine tree growth promoter was sprayed gently on the needles and stems with a suitable spraying speed, so that the...

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Abstract

The invention relates to a pine tree endophytic bacterium FBS135. The pine tree endophytic bacterium FBS135 belongs to the Pantoea eucalypti genus, and is preserved in China Center for Type Culture Collection in Wuhan University, the preservation number is CCTCC NO:M2019198, and the preservation date is 9th April, 2019. The 16S rDNA sequence of the pine tree endophytic bacterium FBS135 is shown asSEQ ID NO.1. The pine tree endophytic bacterium FBS135 has a promoting function on pine tree growth, test data shows that a pine tree growth accelerant prepared from the bacterium has an obvious promoting function on various growth indexes such as the roots, stems and leaves of pine tree seedlings of pine tree seedlings, the system stress resistance of the pine tree seedlings can be improved, andinfection of pathogenic bacteria and the probability of attacking death in an adverse situation are reduced.

Description

technical field [0001] The invention relates to a pine endophytic bacterium and its application, belonging to the technical field of microbes. Background technique [0002] Pine trees are very tolerant of poor, dry soils and are often bred as "pioneers", so they can be widely distributed around the world. As a member of Pinaceae, Pinus massoniana is the main species of Chinese plantations. Not only that, masson pine also has huge economic value. For example, due to its short growth period, the masson pine can provide a large amount of wood raw materials for the papermaking and woodworking industries every year; the rosin extracted from the masson pine is also a very important chemical raw material; Additives are also often used in chemical, pharmaceutical and other industrial production fields; in addition, pine trees have the characteristics of evergreen in all seasons, and are also widely used in the field of garden landscape in our country. [0003] However, pine trees...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12R2001/01C12N1/205
Inventor 樊奔陆一鸣宋忠文
Owner NANJING FORESTRY UNIV
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