Fermentation culture medium and method for culturing bacterial cellulose membrane

A technology of bacterial cellulose film and fermentation medium, which is applied in the field of fermentation medium and bacterial cellulose film cultivation, can solve the problems of low transparency and inability to directly observe wound conditions, achieve good application prospects and reduce high temperature sterilization. link, cost-saving effect

Inactive Publication Date: 2019-07-09
ZHENDE MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] General wound dressings such as gauze, non-woven cloth and foam dressings, etc., are not transparent due to their material properties, so the wound cannot be directly observed
If the wound is infected or secondary injury occurs, the wound cannot be detected in time through direct observation, which will have a certain impact on the patient.

Method used

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  • Fermentation culture medium and method for culturing bacterial cellulose membrane
  • Fermentation culture medium and method for culturing bacterial cellulose membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Culture medium preparation: Add 33g of carbon source (20g of glucose, 8g of sucrose and 5g of glycerol) per liter of medium, 24g of nitrogen source (10g of peptone, 7g of corn steep liquor, 7g of ammonium sulfate), 1.5g of inorganic salt (chloride Sodium 0.4g, potassium chloride 0.1g, calcium chloride 0.2g, magnesium sulfate 0.1g, sodium dihydrogen phosphate 0.3g, potassium dihydrogen phosphate 0.4g), sodium hydroxide 2g, citric acid 0.8g and acetic acid 3ml Fermentation medium, spare.

[0042] (2) pH adjustment: adjust the pH of the medium to 4.2 with acetic acid.

[0043] (3) Inoculation: Acetobacter xylinum was inserted into the above medium at an inoculation amount of 6% (accounting for the medium), and stirred.

[0044] (4) Quantitatively 500ml was poured into a shallow plate, poked with a plastic wrap to ventilate the seal, and cultured statically for 8 days at 28°C to obtain a bacterial cellulose membrane without bacterial contamination. After washing and dr...

Embodiment 2

[0046] (1) Culture medium preparation: Add 33g of carbon source (20g of glucose, 8g of sucrose and 5g of glycerol) per liter of medium, 24g of nitrogen source (10g of peptone, 7g of corn steep liquor, 7g of ammonium sulfate), 1.5g of inorganic salt (chloride Sodium 0.4g, potassium chloride 0.1g, calcium chloride 0.2g, magnesium sulfate 0.1g, sodium dihydrogen phosphate 0.3g, potassium dihydrogen phosphate 0.4g), sodium hydroxide 2g, citric acid 0.8g and acetic acid 3ml Fermentation medium, spare.

[0047] (2) pH adjustment: adjust the pH of the medium to 5.2 with acetic acid.

[0048](3) Inoculation: Acetobacter xylinum was inserted into the above medium at an inoculation amount of 6% (accounting for the medium), and stirred.

[0049] (4) Quantitatively 500ml was poured into a shallow dish, poked holes with plastic wrap for air-permeable sealing, and cultured statically for 8 days at 28°C, all of which were contaminated with bacteria, and bacterial cellulose membranes could n...

Embodiment 3

[0051] (1) Culture medium preparation: Add 33g of carbon source (20g of glucose, 8g of sucrose and 5g of glycerol) per liter of medium, 24g of nitrogen source (10g of peptone, 7g of corn steep liquor, 7g of ammonium sulfate), 1.5g of inorganic salt (chloride Sodium 0.4g, potassium chloride 0.1g, calcium chloride 0.2g, magnesium sulfate 0.1g, sodium dihydrogen phosphate 0.3g, potassium dihydrogen phosphate 0.4g), sodium hydroxide 2g, citric acid 0.8g and acetic acid 3ml Fermentation medium, spare.

[0052] (2) pH adjustment: adjust the pH of the medium to 4.0 with acetic acid.

[0053] (3) Inoculation: insert Acetobacter xylinum into the above-mentioned culture medium at an inoculum amount of 20% (accounting for the culture medium), and stir.

[0054] (4) Quantitatively 500ml was poured into a shallow plate, poked with a plastic wrap to ventilate the seal, and cultured statically for 5 days at 28°C to obtain a bacterial cellulose membrane without bacterial contamination. After...

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Abstract

The invention discloses a fermentation culture medium and a method for culturing a bacterial cellulose membrane. The formula of the fermentation culture medium involves that 33 grams of a carbon source, 24 grams of a nitrogen source, 1.5 grams of inorganic salt, 2 grams of sodium hydroxide, 0.8 gram of citric acid and 3 milliliters of acetic acid are added into each liter of the culture medium. The fermentation culture medium and the method for culturing the bacterial cellulose membrane have the advantages that the formula of the culture medium cooperates with a method that the acetic acid isadded to regulate the pH value of the fermentation culture medium to achieve the antibacterial effect without sterilization; when the pH value is adjusted to 4.0-4.2, the obtained product has excellent solution absorption performance after cleaning and water pressurizing, improves the use comfortable feeling and accelerates wound healing; the obtained product has excellent transparency after beingcleaned, provides convenience for observing the wound situation, can serve as a visible wound healing dressing, and has good application prospects in wound surface dressings.

Description

technical field [0001] The invention relates to the field of bacterial cellulose production, in particular to a fermentation medium and a method for cultivating bacterial cellulose film. Background technique [0002] Bacterial Cellulose (BC) is a general term for natural cellulose produced by bacterial fermentation. It is produced by the fermentation of certain microorganisms in the genera Acetobacter, Agrobacterium, Rhizobium and Sarcina, and its typical representative bacteria is Gluconacetobacter xylinum ( Glucoacetobacter xylinum). Bacterial cellulose and plant cellulose have the same chemical composition, which is a chain polymer formed by D-glucopyranose anhydride connected by β-1,4-glucosidic bonds, but the morphological structure and supramolecular structure of bacterial cellulose are different from those of plant cellulose. Plant cellulose is very different and belongs to the typical bio-nanofibrous material. At present, the production of bacterial cellulose by m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01
CPCC12P19/04
Inventor 陆潮峰鲁建国丁海微曹黎明王姣
Owner ZHENDE MEDICAL CO LTD
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